■亚甲基四氢叶酸还原酶(MTHFR)基因是叶酸-高半胱氨酸代谢中的必需基因。最近,在COVID-19感染的随访中,发现同型半胱氨酸水平是一个重要标志物。因此,本研究旨在检测遗传多态性对单核苷酸多态性(SNPs)的影响(c.66A>G,c.1298A>C,和c.677CT)关于COVID-19感染。
■在Al-Shifa(巴格达,伊拉克)从2020年11月到2021年3月。使用四引物扩增难治性突变系统-聚合酶链反应(ARMS-PCR)技术检测SNP的基因型。比值比(OR)用于检测SNP与COVID-19感染之间的关系。通过SHEsis软件进行单倍型分析。
■轻度/中度病例与重度/危重病例之间存在显着差异(35-45岁),(46-55),和(56-65)年(分别为P<0.0001,P=0.01和P=0.006)。结果显示T等位基因对于SNPc.677>C有显著差异(P<0.0001,OR=4.58)。SNPc.1298A>C的C等位基因表示显著差异(P<0.001,OR=3.15)。此外,SNPc.677C>T的G等位基因显示显着差异(P<0.001,OR=6.64)。因此,这些SNP显示出发展COVID-19感染的易感性。关于C-A-A,T-A-A和T-C-G单倍型表明对照组和患者组之间存在显著差异。C-A-A与风险降低有关,并表明对COVID-19感染发展具有保护作用(P<0.0001,OR=0.218)。风险增加与T-A-A和T-C-G单倍型相关,并表明对COVID-19感染发展的风险影响(分别为P<0.0001,P=0.004,OR=15.5,OR=6.772)。此外,研究了SNP的连锁不平衡(LD),完整的D'值为99。
■SNP的遗传多态性(c.66A>G,c.1298A>C,和c.677C>T)在伊拉克人群中与COVID-19感染有关。
UNASSIGNED: The methylenetetrahydrofolate reductase (MTHFR) gene is an essential gene in the metabolism of folate-homocysteine. Recently, the level of homocysteine was found to be a significant marker in the follow-up of COVID-19 infection. Thus, this study aimed to detect the effect of genetic polymorphisms for single nucleotide polymorphisms (SNPs) (c.66A>G, c.1298A>C, and c.677CT) on COVID-19 infection.
UNASSIGNED: Blood samples were collected from 270 patients with COVID-19 in the medical center of Al-Shifa (Baghdad, Iraq) from November 2020 to March 2021. Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for the detection of genotypes of SNPs. The odds ratio (OR) was used to detect the relationship between SNPs and COVID-19 infections. Haplotype analysis was performed by SHEsis software.
UNASSIGNED: There was a significant difference between mild/moderate cases and severe/critical cases for ages (35-45), (46-55), and (56-65) years (P<0.0001, P=0.01, and P=0.006, respectively). The results showed significant differences in the T allele for SNP c.677>C (P<0.0001 and OR=4.58). The C allele for SNP c.1298A>C indicated significant differences (P<0.001 and OR=3.15). Besides, the G allele for SNP c.677C>T showed significant differences (P<0.001 and OR=6.64). Consequently, these SNPs showed a predisposition to the development of COVID-19 infection. With regard to the C-A-A, T-A-A and T-C-G haplotypes indicated significant differences between the control and patient groups. The C-A-A was related to a decreased risk and indicated a protective effect against COVID-19 infection development (P<0.0001 and OR=0.218). The increased risk was associated with T-A-A and T-C-G haplotypes and indicated the risk impact on COVID-19 infection development (P<0.0001, P=0.004, and OR=15.5, OR=6.772, respectively). Furthermore, the linkage disequilibrium (LD) for SNPs was studied, and the complete D\' value was 99.
UNASSIGNED: The genetic polymorphisms of SNPs (c.66A>G, c.1298A>C, and c.677C>T) in the Iraqi population were associated with COVID-19 infection.