背景:治疗由产生生物膜的病原体和人类癌症引起的感染的无效性对全球公共卫生组织提出了相当大的挑战。为了解决这个问题,我们的研究重点是探索合成新的Co(II)配合物的潜力,Cu(II),Ni(II),和锌(II)与山梨酸,以增强其抗菌,抗生物膜,和抗癌特性。
方法:通过山梨酸与Co(II)反应合成了四种新型配合物作为固相,Cu(II),Ni(II),和Zn(II)。这些配合物的特点是通过各种技术,包括红外光谱,紫外可见光谱,质子核磁共振(1HNMR),和热分析技术,包括热重分析(TG)。
结果:从所有研究的化学表征方法获得的数据证实了山梨酸盐金属络合物的化学结构。这些复合物对革兰氏阳性和革兰氏阴性细菌均表现出抗菌和抗生物膜特性。此外,这些复合物增强了常用抗生素的抗菌作用,如庆大霉素和亚胺培南,分数抑制浓度(FIC)指数≤0.5。值得注意的是,Cu(II)配合物显示出最有效的抗菌和抗生物膜活性,蜡样芽孢杆菌和大肠杆菌的最小抑菌浓度(MIC)值分别为312.5µg/mL和625.0µg/mL,分别。此外,使用甲基噻唑基四唑(MTT)方法的体外测定显示,在用所研究的金属配合物处理后,对人结肠癌细胞系(HCT-116细胞)的生长具有抑制作用。Co(II)的IC50值,Cu(II),Zn(II),Ni(II)为3230µg/mL,2110微克/毫升,3730µg/mL,和2240微克/毫升,分别。
结论:我们的发现为制药公司探索开发涉及传统抗生素或抗癌药物与山梨酸铜络合物的新型组合提供了潜力。
BACKGROUND: The ineffectiveness of treatments for infections caused by biofilm-producing pathogens and human carcinoma presents considerable challenges for global public health organizations. To tackle this issue, our study focused on exploring the potential of synthesizing new complexes of Co(II), Cu(II), Ni(II), and Zn(II) with sorbic acid to enhance its antibacterial, antibiofilm, and anticancer properties.
METHODS: Four novel complexes were synthesized as solid phases by reacting sorbic acid with Co(II), Cu(II), Ni(II), and Zn(II). These complexes were characterized by various technique, including infrared spectra, UV-Visible spectroscopy, proton nuclear magnetic resonance (1H NMR), and thermal analysis techniques, including thermogravimetry (TG).
RESULTS: The data acquired from all investigated chemical characterization methods confirmed the chemical structure of the sorbate metal complexes. These complexes exhibited antibacterial and antibiofilm properties against both Gram-positive and Gram-negative bacteria. Furthermore, these complexes enhanced the antibacterial effects of commonly used antibiotics, such as gentamicin and imipenem, with fractional inhibitory concentration (FIC) indices ≤ 0.5. Notably, the Cu(II) complex displayed the most potent antibacterial and antibiofilm activities, with minimum inhibitory concentration (MIC) values of 312.5 µg/mL and 625.0 µg/mL for Bacillus cereus and Escherichia coli, respectively. Additionally, in vitro assays using the methyl thiazolyl tetrazolium (MTT) method showed inhibitory effects on the growth of the human colon carcinoma cell line (HCT-116 cells) following treatment with the investigated metal complexes. The IC50 values for Co(II), Cu(II), Zn(II), and Ni(II) were 3230 µg/mL, 2110 µg/mL, 3730 µg/mL, and 2240 µg/mL, respectively.
CONCLUSIONS: Our findings offer potential for pharmaceutical companies to explore the development of novel combinations involving traditional antibiotics or anticancer drugs with sorbate copper complex.