在真核细胞中,膜接触位点(MCSs)通过使膜紧密靠近而不融合来介导细胞器之间的相互作用和通讯。这些位点在细胞内运输中起着至关重要的作用,信号转导,和细胞器功能的调节。在最近的一项研究中,我们从出版物中汇编了MCS蛋白质和复合物的数据,以创建MCSdb数据库。在数据编译期间,我们发现很多MCSs,它们相关的蛋白质,和复合物与巨自噬/自噬高度相关。为了阐明MCSs在自噬中的作用,我们从MCSdb重组了自噬相关的MCS蛋白和复合物,创建名为AutoMCSNavigator的数据映射。此图的当前版本包括30种复合物和84种蛋白质,涵盖13种不同的MCSs和7种。同时,我们在MCSdb网站上嵌入了AutoMCSNavigator的专用网页。这个网页有一个精心策划的视觉指南,分层显示MCS蛋白质和参与自噬的复合物。总之,我们的研究开发了一个用户友好的视觉地图,用于查询,浏览,和可视化的自噬相关的MCS蛋白和复合物的详细信息。该工具使研究人员可以轻松了解与自噬相关的MCS结构,装配,功能,以及相关疾病的治疗策略。AutoMCSNavigator可在https://cellknowledge.com上免费获得。cn/mcsdb/自噬。html.
In eukaryotic cells, membrane contact sites (MCSs) mediate interactions and communication between organelles by bringing their membranes into close proximity without fusion. These sites play crucial roles in intracellular transport, signal transduction, and the regulation of organelle functions. In a recent study, we compiled data on MCS proteins and complexes from publications to create the MCSdb database. During data compilation, we discovered that many MCSs, their associated proteins, and complexes are highly relevant to macroautophagy/autophagy. To elucidate the role of MCSs in autophagy, we reorganized the autophagy-related MCS proteins and complexes from MCSdb, creating a data map called AutoMCS Navigator. The current version of this map includes 30 complexes and 84 proteins, covering 13 different MCSs and 7 species. Meanwhile, we embedded a dedicated webpage for AutoMCS Navigator on the MCSdb website. This webpage features an orchestrated visual guide that hierarchically displays MCS proteins and complexes involved in autophagy. In summary, our research has developed a user-friendly visual map for querying, browsing, and visualizing detailed information on autophagy-related MCS proteins and complexes. This tool offers researchers easy access to understand autophagy-related MCS structure, assembly, functions, and therapeutic strategies for related diseases. AutoMCS Navigator is freely available at https://cellknowledge.com.cn/mcsdb/autophagy.html.