大肠杆菌中质粒介导的喹诺酮耐药(PMQR)基因和移动粘菌素耐药(MCR)基因(E。大肠杆菌)已被广泛鉴定,这被认为是对公共卫生的全球威胁。在本研究中,我们对MCR基因(mcr-1,mcr-2,mcr-3,mcr-4和mcr-5)和PMQR基因进行了分析[qnrA,qnrB,qnrC,qnrD,qnrE1,qnrVC,qnrS,aac(6\')-Ib-cr,qepA,和oqxAB]在来自中国的大肠杆菌中,1993-2019年。从检查的3663个大肠杆菌分离物中,1,613(44.0%)的PMQR基因检测呈阳性,无论是单独或组合。同时,262个分离株(7.0%)携带MCR基因。对携带MCR基因的菌株的17种抗生素的最小抑制浓度(MIC)分析显示出普遍的多药耐药性。对多粘菌素的抗性在4μg/mL至64μg/mL之间变化,MIC50和MIC90分别为8μg/mL和16μg/mL,分别。此外,这些耐药基因检出率的波动与抗生素政策的出台相关,宿主起源,时间趋势,和地理分布。为了实施控制和预防策略,需要对细菌中的PMQR和MCR变体进行连续监测。
Plasmid-mediated quinolone resistance (PMQR) genes and mobile colistin resistance (MCR) genes in Escherichia coli (E. coli) have been widely identified, which is considered a global threat to public health. In the present study, we conducted an analysis of MCR genes (mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5) and PMQR genes [qnrA, qnrB, qnrC, qnrD, qnrE1, qnrVC, qnrS, aac(6\')-Ib-cr, qepA, and oqxAB] in E. coli from China, 1993-2019. From the 3,663 E. coli isolates examined, 1,613 (44.0%) tested positive for PMQR genes, either individually or in combination. Meanwhile, 262 isolates (7.0%) carried the MCR genes. Minimum inhibitory concentration (MIC) analyses of 17 antibiotics for the MCR gene-carrying strains revealed universal multidrug resistance. Resistance to polymyxin varied between 4 μg/mL and 64 μg/mL, with MIC50 and MIC90 at 8 μg/mL and 16 μg/mL, respectively. In addition, fluctuations in the detection rates of these resistant genes correlated with the introduction of antibiotic policies, host origin, temporal trends, and geographical distribution. Continuous surveillance of PMQR and MCR variants in bacteria is required to implement control and prevention strategies.