PDF(Pubmed)
Abstract：
OBJECTIVE: Cronobacter is an emerging foodborne opportunistic pathogen, which can cause neonatal meningitis, bacteremia, and NEC by contaminating food. However, the entire picture of foodborne Cronobacter carriage of the mcr genes is not known. Here, we investigated the mcr genes of Cronobacter isolates by whole-genome sequencing and found 133 previously undescribed Cronobacter isolates carrying mcr genes. Further genomic analysis revealed that these mcr genes mainly belonged to the mcr-9 and mcr-10. Genomic analysis of the flanking structures of mcr genes revealed that two core flanking structures were prevalent in foodborne Cronobacter isolates, and the flanking structure carrying IS1R was found for the first time in this study.
摘要：
抗生素耐药性已成为全球性的公共卫生和生物安全问题。粘菌素的临床疗效受到第一个移动粘菌素抵抗（mcr）基因的报道的危害，随后,已鉴定出mcr-1至mcr-10的mcr基因家族。Cronobacter是一种新兴的食源性机会致病菌,会导致新生儿脑膜炎，菌血症,和坏死性小肠结肠炎（NEC）污染的食物。然而,食源性Cronobacter携带mcr基因的全貌尚不清楚。这里,我们通过全基因组测序研究了Cronobacter分离株(N=877)的mcr基因.我们发现877种先前未描述的Cronobacter分离物中有133种携带mcr基因。进一步的基因组分析表明，这些mcr基因主要属于mcr-9和mcr-10。对mcr基因侧翼结构的基因组分析显示，两个核心侧翼结构在食源性Cronobacter分离株中普遍存在，在这项研究中首次发现了带有IS1R的侧翼结构。这些发现表明，携带mcr基因的食源性Cronobacter分离株已经对人类健康构成威胁。重要性Cronobacter是一种新兴的食源性机会致病菌，会导致新生儿脑膜炎，菌血症,和NEC污染食物。然而,食源性Cronobacter携带mcr基因的全貌尚不清楚。这里,我们通过全基因组测序研究了Cronobacter分离株的mcr基因，发现133个以前未描述的Cronobacter分离株携带mcr基因。进一步的基因组分析表明，这些mcr基因主要属于mcr-9和mcr-10。对mcr基因侧翼结构的基因组分析显示，两个核心侧翼结构在食源性Cronobacter分离株中普遍存在，在这项研究中首次发现了带有IS1R的侧翼结构。
