哺乳动物的卵巢已经开发出专门的机制来防止病原体感染;然而,对甲壳类动物卵巢先天免疫系统的了解仍然有限。为了阐明卵巢对病毒挑战的防御机制,我们对东方河虾(日本沼虾)进行聚I:C,模拟病毒dsRNA的双链RNA类似物,并分析了卵巢的转录组概况。同时,在肝胰腺上进行RNA-seq分析,公认的免疫相关组织,以下polyI:C挑战以研究卵巢和肝胰腺的不同反应机制,并全面了解两种组织的免疫反应。结果表明,1368个基因在卵巢中差异表达,其中903个基因上调,465个基因下调。随后的分析表明,这些差异表达基因(DEG)包括许多与先天免疫相关的基因,例如C型凝集素的成员,纤维蛋白原相关蛋白(Frep),Toll样受体,和NOD样受体(NLR)基因家族,以及酸性磷酸酶,清道夫受体,Crustin,唐氏综合征细胞粘附分子(Dscam),血蓝蛋白,和脂多糖和β-1,3-葡聚糖结合蛋白(LGBP)。此外,DEGs包括几个与卵巢发育有关的基因,如Sox8,卵黄蛋白原,前颗粒蛋白,细胞周期蛋白依赖性激酶,蜕皮激素受体,卷曲,和福克斯基因家族的成员。在肝胰腺中,总共确定了729个DEG。两种组织中DEGs的比较表明,两组中只有91个基因是共有的,突出显示对聚I:C刺激的显著组织特异性反应。这项研究旨在增强我们对卵巢应对病原体暴露所采用的免疫保护机制的理解,并为研究甲壳类动物的卵巢生殖免疫奠定基础。
The ovary in mammals has developed specialized mechanisms for protection against pathogen infections; however, the understanding of the innate immune system in the ovary of crustaceans is still limited. To elucidate the ovary\'s defense mechanisms in response to viral challenges, we subjected oriental river prawns (Macrobrachium nipponense) to poly I:C, a double-stranded RNA analog that emulates viral dsRNA, and analyzed the ovary\'s transcriptome profiles. Concurrently, RNA-seq analysis was performed on the hepatopancreas, a well-recognized immune-related tissue, following poly I:C challenge to investigate the distinct response mechanisms of the ovary and hepatopancreas and to gain a comprehensive understanding of the immune responses in both tissues. The results indicate that 1368 genes are differentially expressed in the ovary, with 903 genes upregulated and 465 genes downregulated. Subsequent analysis reveals that these differentially expressed genes (DEGs) include numerous genes associated with innate immunity, such as members of the C-type lectin, fibrinogen-related protein (Frep), Toll-like receptor, and NOD-like receptor (NLR) gene families, as well as acid phosphatase, scavenger receptor, crustin, Down syndrome cell adhesion molecule (Dscam), hemocyanin, and lipopolysaccharide and beta-1,3-glucan binding protein (LGBP). Furthermore, the DEGs include several genes related to ovary development, such as sox8, vitellogenin, progranulin, cyclin-dependent kinase, ecdysone receptor, frizzled, and members of the Fox gene family. In the hepatopancreas, a total of 729 DEGs were identified. Comparison of the DEGs in both tissues indicates that only 91 genes are common to both groups, highlighting significant tissue-specific responses to poly I:C stimulation. This study aims to enhance our understanding of the immune protective mechanisms employed by the ovary in response to pathogen exposure and establishes a foundation for investigating ovarian reproductive immunity in crustaceans.