Abstract：
Tropomyosin (TM) is the main allergen of Macrobrachium nipponense. Recombinant allergens have great prospects in the detection, diagnosis, and treatment of food allergens. The purpose of this study was to compare the differences in structure and allergenicity between natural TM and recombinant TM. Recombinant TM of M. nipponense with a molecular weight of 38 kDa was successfully expressed in the Escherichia coli system. The amino acid sequence as well as secondary structure between natural and recombinant TM were similar, which were verified by mass and CD spectrometry, respectively. Studies showed that both natural TM and recombinant TM had strong allergenicity, and recombinant TM was more allergenic, which could be used as a substitute for natural TM in the diagnosis and treatment of shrimp allergy. This study provided stable and reliable allergen components for the detection of crustacean allergens and the diagnosis and treatment of food allergies caused by crustacean allergens.
摘要：
原肌球蛋白（TM）是日本沼虾的主要过敏原。重组过敏原在检测中具有很大的应用前景,诊断,和食物过敏原的治疗。本研究的目的是比较天然TM和重组TM在结构和致敏性上的差异。在大肠杆菌系统中成功表达了分子量为38kDa的日本M.nipponense重组TM。天然和重组TM的氨基酸序列和二级结构相似，通过质谱和CD光谱验证，分别。研究表明，天然TM和重组TM均具有较强的致敏性，重组TM更容易过敏，可作为天然TM的替代品用于对虾过敏的诊断和治疗。本研究为甲壳类过敏原的检测和甲壳类过敏原引起的食物过敏的诊断和治疗提供了稳定可靠的过敏原成分。
