国际研究人员已经注意到甲基叔丁基醚(MTBE)在不同营养水平上的不利影响。然而,关于其对蚯蚓氧化应激和DNA损伤的影响的证据尚不清楚.在这项研究中,蚯蚓在不同剂量的MTBE(0.0毫克/千克,10.0mg/kg,30.0mg/kg,和60.0mg/kg)污染的农业土壤持续7天,14天,21天,28天,分别。结果表明,与对照组相比,MTBE处理组蚯蚓的活性氧(ROS)含量显着增加。在MTBE治疗组中,超氧化物歧化酶的活性,过氧化氢酶,过氧化物酶,谷胱甘肽S-转移酶在暴露7天时被显著激活,增长了36.3-78.9%,51.8-97.3%,36.5-61.9%,和12.0-54.8%,分别。然后,这些防御酶的活性随着暴露时间和MTBE浓度的变化而显示出各种变化。特别是在60.0mgkg-1组中,抗氧化酶和GST在暴露14天时仍被显著激活,然后在暴露28天时被显著抑制。橄榄尾矩分析显示,在接触28天时,10.0mgkg-1组的DNA损伤明显,30.0mg/kg和60.0mg/kg组在暴露7天时发现了这种损害。这一结果与蚯蚓体内丙二醛的积累是一致的。此外,对IBRv2的分析表明,MTBE处理对蚯蚓的影响具有剂量和时间依赖性。该研究有助于更好地了解MTBE对土壤无脊椎动物的影响,为MTBE应用的土壤保护提供理论支持。
Adverse effects of methyl tertiary-butyl ether (
MTBE) have been noticed at different trophic levels by international researchers. However, there was unclear evidence about its effects on oxidative stress and DNA damage in earthworms. In this study, earthworms were cultivated in various doses of
MTBE (0.0 mg/kg, 10.0 mg/kg, 30.0 mg/kg, and 60.0 mg/kg) contaminated agricultural soil for 7 days, 14 days, 21 days, and 28 days, respectively. The result showed that the reactive oxygen species (ROS) content of earthworms significantly increased in MTBE treatment groups compared to the control group. In
MTBE treatment groups, the activities of superoxide dismutase, catalase, peroxidase, and glutathione S-transferase were significantly activated at the exposure of 7 days, which increased by 36.3-78.9%, 51.8-97.3%, 36.5-61.9%, and 12.0-54.8%, respectively. Then, the activities of these defense enzymes showed various changes following the changes in exposure times and MTBE concentrations. Especially in the 60.0 mg kg-1 group, both antioxidant enzymes and GST were still significantly activated at the exposure of 14 days and then significantly inhibited at the exposure of 28 days. The analysis of olive tail moment showed significant DNA damage in the 10.0 mg kg-1 group at the exposure of 28 days, and this damage in 30.0 mg/kg and 60.0 mg/kg groups was found at the exposure of 7 days. This result was consistent with the malondialdehyde accumulation in earthworms. Additionally, the analysis of IBRv2 showed the effects of
MTBE treatments on earthworms in dose- and time-dependent manners. This study helps better to understand the effects of MTBE on soil invertebrate animals and provide theoretical support for soil protection in governing
MTBE application.