The extensive use of methyl tert-butyl ether (MTBE) as a gasoline additive has caused serious environmental problems that need to be addressed urgently. The feasibility of remediation of MTBE-contaminated groundwater by ZSM-5 zeolite with SiO2/Al2O3 ratio of 50/130/360 was explored. The SiO2/Al2O3 ratio had a great influence on the physicochemical properties and structure, as well as the adsorption and mass transfer of MTBE on ZSM-5. The adsorption of MTBE on zeolites with SiO2/Al2O3 ratios of 50 and 130/360 followed the Langmuir and Freundlich models, respectively, and was controlled by different mass transfer processes. The morphology and adsorption capacity of ZSM-5 (50) and ZSM-5 (130) differed significantly, while the differences between ZSM5-(130) and ZSM-5 (360) were less pronounced. ZSM-5 (360) had higher adsorption capacity and adsorption efficiency for MTBE, and the larger BET surface area, pore volume and stronger hydrophobicity were the key factors to promote MTBE adsorption. Compared to activated carbon (AC), ZSM-5 (360) was more effective for MTBE removal at low concentrations (≤200 mg·L-1) and had the advantage of selective adsorption of MTBE with the addition of BTEX. In column adsorption, decreasing the concentration had opposite effects on MTBE removal by ZSM-5 and AC. At 5-10 mg·L-1, ZSM-5 (360) column reduced effluent concentration and improved bed utilization and removal efficiency.

Sphingolipids, including sphingosine and sphinganine, are one of the major classes of lipids. They serve as constituents of cell membranes and lipid rafts and aid in the performance of cell-cell communication and adhesion. Abnormal levels of sphingolipids in the aqueous humor can indicate impaired sphingolipid metabolism and associated ocular pathologies. Sphingolipids can be extracted from the aqueous humor by the methyl-tert-butyl ether (MTBE) lipid extraction method and subsequently analyzed by liquid chromatography-mass spectrometry (LC-MS). This chapter describes a modified protocol for an MTBE lipid extraction from the aqueous humor, followed by analysis with ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS).

BACKGROUND: Gasoline-powered vehicles and equipment are an important source of air pollution in California. Many gasoline-related pollutants pose significant health concerns. The California Air Resources Board strictly regulates the state\'s gasoline formulation and vehicle emissions.
OBJECTIVE: To investigate exposure trends for gasoline-related air pollutants between 1996 and 2014, capturing the period before and after the removal of methyl t-butyl ether (MTBE).
METHODS: We identified gasoline-related chemicals with known or suspected health concerns and adequate ambient air monitoring data. Average exposures to the general public were estimated from 1996 to 2014 in five major air basins and statewide. We determined the fractions of exposures attributable to gasoline use and evaluated cancer and non-cancer risks for chemicals with available cancer potencies and health reference values.
RESULTS: We found that average gasoline-attributable cancer risks for the general California population from the most highly emitted carcinogens (acetaldehyde, benzene, 1,3-butadiene, and formaldehyde) declined by over 80% between 1996 and 2014. This decline occurred despite roughly constant statewide gasoline sales, an increase in vehicle miles traveled, and an approximately 10% increase in vehicle registrations over this same period. Naphthalene, measured as a volatile organic compound (VOC), was the most abundant gasoline-related polycyclic aromatic hydrocarbon (PAH). From 1996 to 2014, gasoline-attributable cancer risks for naphthalene were estimated to drop approximately threefold in the South Coast Air Basin. Exposures to gasoline-related chemicals associated with non-cancer health effects, such as chronic respiratory toxicity or neurotoxicity, were generally below levels of concern. The exception was acrolein, with gasoline-related exposures in 2014 estimated to be high enough to pose risks for respiratory toxicity.
UNASSIGNED: Our historical analysis demonstrated the success of California\'s regulatory efforts to reduce gasoline-related air pollutant exposures and risks to the general public. New efforts are focused on addressing gasoline-related and other air pollution in heavily impacted communities affected by multiple environmental and social stressors.

In this work, a new type of composite nanoparticles, \'pearl chain\', was developed by linking titanium dioxide and silicon dioxide by polyacrylic acid polymer chains, and the prepared TiO2-PAA-SiO2 composite nanoparticles were analysed by SEM, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy and thermogravimetric analysis, zeta potential, x-ray diffraction, etc. The success of this work was verified by the successful linking of TiO2-PAA-SiO2 composite nanoparticles.TiO2-PAA-SiO2 composite nanoparticles were analysed to verify the successful attachment of pearl chains. The obtained TiO2-PAA-SiO2 were subsequently blended in different ratios to prepare polyvinylidene fluoride (PVDF) ultrafiltration membranes. The membrane performance was tested by porosity and water contact angle measurements, scanning electron microscopy, as well as experiments using bovine serum proteins and MTBE interception. The results showed that when a certain amount of TiO2-PAA-SiO2 was added, the surface wettability, porosity and permeability of the prepared modified composite membranes were significantly improved, and the BSA adsorption rate was increased from 71.59% to 80.86%, and the retention rate of MTBE was increased by 77%, in addition to showing a better anti-pollution effect (FRR: 91.07%). It was finally concluded that the prepared membranes embedded with 1.0 wt.% TiO2-PAA-SiO2 nanofillers showed good overall filtration performance, better contamination resistance and remarkable durability. The present work successfully demonstrated the feasibility of using polyacrylic acid chemical chains to connect nanoparticles with different functions to prevent particle loss and substantially enhance membrane performance, which is valuable for bridging connection of composite nanoparticles and exploring the development of high-performance ultrafiltration membranes.

Fatty acids (FAs) are used, often in combination with stable isotopes (SIs), as chemical biomarkers to assess the contribution of different prey to the diet of consumers and define food web structure and dynamics. Extraction of lipids is traditionally carried out using methanol (MeOH) combined with chloroform or dichloromethane, these latter being well-known environmental pollutant and potential carcinogenic agents. Recently, extraction protocols based on methyl tert-butyl ether (MTBE) and MeOH have been proposed as an alternative to halogenated solvents in lipidomic studies. However, no specific investigation has been performed to assess MTBE suitability in marine ecological studies including FA analysis together with SI measurements. We used an analytical workflow for qualitative and quantitative analysis of FAs and SIs in field samples of phytoplankton, zooplankton and the scyphomedusa Pelagia noctiluca, applying MTBE in comparison with chloroform- and dichloromethane-based protocols for total lipid extraction. Our analysis suggested that MTBE is a reliable substitute for lipid extraction in trophic ecology studies in marine planktonic organisms.

The degradation of the prevalent environmental contaminants benzene, toluene, ethylbenzene, and xylenes (BTEX) along with a common co-contaminant methyl tert-butyl ether (MTBE) by Rhodococcus rhodochrous ATCC Strain 21198 was investigated. The ability of 21198 to degrade these contaminants individually and in mixtures was evaluated with resting cells grown on isobutane, 1-butanol, and 2-butanol. Growth of 21198 in the presence of BTEX and MTBE was also studied to determine the growth substrate that best supports simultaneous microbial growth and contaminants degradation. Cells grown on isobutane, 1-butanol, and 2-butanol were all capable of degrading the contaminants, with isobutane grown cells exhibiting the most rapid degradation rates and 1-butanol grown cells exhibiting the slowest. However, in conditions where BTEX and MTBE were present during microbial growth, 1-butanol was determined to be an effective substrate for supporting concurrent growth and contaminant degradation. Contaminant degradation was found to be a combination of metabolic and cometabolic processes. Evidence for growth of 21198 on benzene and toluene is presented along with a possible transformation pathway. MTBE was cometabolically transformed to tertiary butyl alcohol, which was also observed to be transformed by 21198. This work demonstrates the possible utility of primary and secondary alcohols to support biodegradation of monoaromatic hydrocarbons and MTBE. Furthermore, the utility of 21198 for bioremediation applications has been expanded to include BTEX and MTBE.

Adverse effects of methyl tertiary-butyl ether (MTBE) have been noticed at different trophic levels by international researchers. However, there was unclear evidence about its effects on oxidative stress and DNA damage in earthworms. In this study, earthworms were cultivated in various doses of MTBE (0.0 mg/kg, 10.0 mg/kg, 30.0 mg/kg, and 60.0 mg/kg) contaminated agricultural soil for 7 days, 14 days, 21 days, and 28 days, respectively. The result showed that the reactive oxygen species (ROS) content of earthworms significantly increased in MTBE treatment groups compared to the control group. In MTBE treatment groups, the activities of superoxide dismutase, catalase, peroxidase, and glutathione S-transferase were significantly activated at the exposure of 7 days, which increased by 36.3-78.9%, 51.8-97.3%, 36.5-61.9%, and 12.0-54.8%, respectively. Then, the activities of these defense enzymes showed various changes following the changes in exposure times and MTBE concentrations. Especially in the 60.0 mg kg-1 group, both antioxidant enzymes and GST were still significantly activated at the exposure of 14 days and then significantly inhibited at the exposure of 28 days. The analysis of olive tail moment showed significant DNA damage in the 10.0 mg kg-1 group at the exposure of 28 days, and this damage in 30.0 mg/kg and 60.0 mg/kg groups was found at the exposure of 7 days. This result was consistent with the malondialdehyde accumulation in earthworms. Additionally, the analysis of IBRv2 showed the effects of MTBE treatments on earthworms in dose- and time-dependent manners. This study helps better to understand the effects of MTBE on soil invertebrate animals and provide theoretical support for soil protection in governing MTBE application.

Cholesterol is an essential lipid molecule for several biological functions including the proper functioning of cell membranes, lipoproteins, and lipid rafts, as well as the synthesis of bile acids, vitamin D, and steroid hormones. Cholesterol can be extracted from liver tissue by multiple methods of lipid extraction. Subsequently, gas chromatography-mass spectrometry (GC-MS) can be used to obtain the highest level of sensitivity and selectivity in the analysis of cholesterol. This chapter describes two methods of lipid extraction for liver tissue, Bligh and Dyer and methyl tertiary butyl ether (MTBE), followed by an analysis with GC-MS.

The co-contamination of metals and organic pollutants, such as Pb and methyl tert-butyl ether (MTBE), in groundwater, has become a common and major phenomenon in many contaminated sites. This study evaluated the feasibility of their simultaneous removal with permeable reactive barrier (PRB) packed with mixed zeolites (clinoptilolite and ZSM-5) using fixed-bed column tests and breakthrough curve modeling. The effect of grain size on the permeability of PRB and removal efficacy was also assessed by granular and power clinoptilolite. The replacement of granular clinoptilolite by powder clinoptilolite largely reduced the breakthrough time but increased the saturation time nearly fourfold. The column adsorption capacity of clinoptilolite powders almost tripled that of clinoptilolite granules (130.6 mg/g versus 45.3 mg/g) due to higher specific surface areas. The minimum thickness and corresponding longevity of PRB were calculated as 7.12 cm and 321.5 min when 5% of granular clinoptilolite was mixed with 5% ZSM-5 and 90% sand as mixed PRB reactive media compared with 10.86 cm and 1230.2 min for the application of powder clinoptilolite. This study is expected to provide theoretical support and guidance for the practical application of mixed adsorbents in PRBs.

Methyl-tert-butyl ether (MTBE) is a fuel oxygenate used in non-United States geographies. Multiple health reviews conclude that MTBE is not a human-relevant carcinogen, and this review provides updated mode of action (MOA), exposure, dosimetry and risk perspectives supporting those conclusions. MTBE is non-genotoxic and has large margins of exposure between blood concentrations at the overall rat 400 ppm inhalation NOAEL and blood concentrations in typical workplace or general population exposures. Non-cancer and threshold cancer hazard quotients range from a high of 0.046 for fuel-pump gasoline station attendants and are 100-1,000-fold lower for general population exposures. Cancer risks conservatively assuming genotoxicity for these same scenarios are all less than 1 × 10-6. The onset of MTBE nonlinear toxicokinetics (TK) in rats at inhalation exposures less than 3,000 ppm, a dose that is also not practically achievable in fuel-use scenarios, indicates that high-dose specific male rat kidney and testes (3,000 and 8,000 ppm) and female mouse liver tumors (8000 ppm) are not quantitatively relevant to humans. Mode of action analyses also indicate MTBE male rat kidney tumors, and lesser so female mouse liver tumors, are not qualitatively relevant to humans. Thus, an integrated analysis of the toxicology, exposure/dosimetry, TK, and MOA data indicates that MTBE presents minimal human cancer and non-cancer risks.