Abstract：
Sphingolipids, including sphingosine and sphinganine, are one of the major classes of lipids. They serve as constituents of cell membranes and lipid rafts and aid in the performance of cell-cell communication and adhesion. Abnormal levels of sphingolipids in the aqueous humor can indicate impaired sphingolipid metabolism and associated ocular pathologies. Sphingolipids can be extracted from the aqueous humor by the methyl-tert-butyl ether (MTBE) lipid extraction method and subsequently analyzed by liquid chromatography-mass spectrometry (LC-MS). This chapter describes a modified protocol for an MTBE lipid extraction from the aqueous humor, followed by analysis with ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS).
摘要：
鞘脂,包括鞘氨醇和鞘氨醇,是脂质的主要类别之一。它们充当细胞膜和脂质筏的成分，并有助于细胞间通讯和粘附的性能。房水中鞘脂的异常水平可以表明鞘脂代谢受损和相关的眼部病变。鞘脂可以通过甲基-叔丁基醚（MTBE）脂质提取方法从房水中提取，然后通过液相色谱-质谱（LC-MS）进行分析。本章描述了从房水中提取MTBE脂质的改良方案，然后用超高效液相色谱-质谱(UHPLC-MS)分析。
