背景:苞片对观赏植物很重要,它们的发育调节过程是复杂的;然而,关于苞片的研究相对较少。在这项研究中,生理,九重葛叶的生化和形态变化,系统地研究了七个发育期的叶芽和苞片。此外,使用PacBio和Illumina测序技术获得了B.glabrabraces的转录组数据,并筛选了调控其发育的关键基因。
结果:扫描电子显微镜显示,通过涉及毛发退化和从绿色到白色的颜色变化的过程来发育苞片。转录组测序显示79,130,973bp的转录物序列和45,788个转录物。差异基因表达分析揭示了七个发育期的50种表达模式,在转录因子如BgAP1,BgFULL,BgCMB1,BgSPL16,BgSPL8,BgDEFA,BgEIL1和BgBH305。生长和发育的KEGG和GO分析显示叶绿素代谢和激素相关的代谢途径参与。叶绿素代谢基因包括BgPORA,BgSGR,BgPPH,BgPAO和BgRCCR。生长激素和脱落酸信号通路涉及44和23个同源基因,和共表达网络分析显示,筛选的基因BgAPRR5和BgEXLA1参与了苞片发育的调控。
结论:这些发现提高了对植物叶片发育的分子机制的认识,为观赏植物生长发育的分子调控和遗传改良提供了重要指导。主要是观赏片。
BACKGROUND: Bracts are important for ornamental plants, and their developmental regulation process is complex; however, relatively little research has been conducted on bracts. In this study, physiological, biochemical and morphological changes in Bougainvillea glabra leaves, leaf buds and bracts during seven developmental periods were systematically investigated. Moreover, transcriptomic data of B. glabra bracts were obtained using PacBio and Illumina sequencing technologies, and key genes regulating their development were screened.
RESULTS: Scanning electron microscopy revealed that the bracts develop via a process involving regression of hairs and a color change from green to white. Transcriptome sequencing revealed 79,130,973 bp of transcript sequences and 45,788 transcripts. Differential gene expression analysis revealed 50 expression patterns across seven developmental periods, with significant variability in transcription factors such as BgAP1, BgFULL, BgCMB1, BgSPL16, BgSPL8, BgDEFA, BgEIL1, and BgBH305. KEGG and GO analyses of growth and development showed the involvement of chlorophyll metabolism and hormone-related metabolic pathways. The chlorophyll metabolism genes included BgPORA, BgSGR, BgPPH, BgPAO and BgRCCR. The growth hormone and abscisic acid signaling pathways involved 44 and 23 homologous genes, and coexpression network analyses revealed that the screened genes BgAPRR5 and BgEXLA1 are involved in the regulation of bract development.
CONCLUSIONS: These findings improve the understanding of the molecular mechanism of plant bract development and provide important guidance for the molecular regulation and genetic improvement of the growth and development of ornamental plants, mainly ornamental bracts.