PDF(Pubmed)
Abstract：
A novel MADS-box transcription factor from Pinus radiata D. Don was characterized. PrMADS11 encodes a protein of 165 amino acids for a MADS-box transcription factor belonging to group II, related to the MIKC protein structure. PrMADS11 was differentially expressed in the stems of pine trees in response to 45° inclination at early times (1 h). Arabidopsis thaliana was stably transformed with a 35S::PrMADS11 construct in an effort to identify the putative targets of PrMADS11. A massive transcriptome analysis revealed 947 differentially expressed genes: 498 genes were up-regulated, and 449 genes were down-regulated due to the over-expression of PrMADS11. The gene ontology analysis highlighted a cell wall remodeling function among the differentially expressed genes, suggesting the active participation of cell wall modification required during the response to vertical stem loss. In addition, the phenylpropanoid pathway was also indicated as a PrMADS11 target, displaying a marked increment in the expression of the genes driven to the biosynthesis of monolignols. The EMSA assays confirmed that PrMADS11 interacts with CArG-box sequences. This TF modulates the gene expression of several molecular pathways, including other TFs, as well as the genes involved in cell wall remodeling. The increment in the lignin content and the genes involved in cell wall dynamics could be an indication of the key role of PrMADS11 in the response to trunk inclination.
摘要：
表征了来自辐射松D.Don的新型MADS-box转录因子。PrMADS11编码一个165个氨基酸的蛋白质，用于属于II组的MADS-box转录因子，与MIKC蛋白结构有关。PrMADS11在早期（1h）响应45°倾斜而在松树的茎中差异表达。拟南芥用35S::PrMADS11构建体稳定转化，以鉴定PrMADS11的推定靶标。大量转录组分析显示947个差异表达基因：498个基因上调，由于PrMADS11的过表达，449个基因下调。基因本体论分析强调了差异表达基因中的细胞壁重塑功能，表明在对垂直茎丢失的反应过程中需要主动参与细胞壁修饰。此外,苯丙素途径也被称为PrMADS11靶标，显示驱动单木素生物合成的基因表达的显着增加。EMSA测定证实PrMADS11与CArG-box序列相互作用。这种TF调节几种分子途径的基因表达，包括其他TFs，以及与细胞壁重塑有关的基因。木质素含量和与细胞壁动力学有关的基因的增加可能表明PrMADS11在对树干倾斜的响应中的关键作用。
