木质纤维素生物质作为生产生物燃料和生物化学品的原料具有非常重要的作用。然而,一个可持续的,高效,并且从这些材料中释放糖的经济竞争性过程仍未实现。在这项工作中,酶水解混合物的优化被评估为一种从轻度预处理的甘蔗渣中最大限度地提取糖的方法。不同的添加剂和酶,包括过氧化氢(H2O2),漆酶,将半纤维素酶和表面活性剂Tween80和PEG4000加入到纤维素分解混合物中,目的是改善生物质水解。增加39%和46%的葡萄糖和木糖浓度,分别,与对照相比(当仅使用纤维素分解混合物(20或35FPUg-1干重)时,当在水解开始时加入H2O2(0.24mM)时获得。另一方面,添加半纤维素酶(81-162μLg-1DM)可将葡萄糖的产量提高到38%,将木糖的产量提高到50%。这项研究的结果表明,可以通过使用补充有添加剂的适当的酶促混合物来增加轻度预处理的木质纤维素生物质中糖的提取。这为更可持续的发展开辟了新的机遇,高效,和经济上具有竞争力的生物质分馏过程。
Lignocellulosic biomasses have a very important role as a raw material to produce biofuels and biochemicals. However, a sustainable, efficient, and economically competitive process for the release of sugars from such materials has still not been achieved. In this work, the optimization of the enzymatic hydrolysis cocktail was evaluated as an approach to maximize sugar extraction from mildly pretreated sugarcane bagasse. Different additives and enzymes, including hydrogen peroxide (H2O2), laccase, hemicellulase and the surfactants Tween 80 and PEG4000 were added to a cellulolytic cocktail with the aim of improving biomass hydrolysis. An increase of 39 % and 46 % of glucose and xylose concentrations, respectively, compared to the control (when only the cellulolytic cocktail (20 or 35 FPU g-1 dry mass), was obtained when H2O2 (0.24 mM) was added at the beginning of the hydrolysis. On the other hand, the addition of hemicellulase (81-162 μL g-1 DM) increased the production of glucose up to 38 % and xylose up to 50 %. The findings of this study reveal that it is possible to increase the extraction of sugars from mildly pretreated lignocellulosic biomass by using an appropriate enzymatic cocktail supplemented with additives. This opens up new opportunities for the development of a more sustainable, efficient, and economically competitive process for biomass fractionation.