■在类风湿关节炎(RA)患者中,骨关节病显着降低自我感知。然而,骨代谢与SPP和细胞活性在分子水平上的内在关系尚不清楚。目的了解RA骨代谢指标与免疫炎症相关蛋白的关系。
■共招募了30名RA患者和30名健康对照。从RA患者和健康对照中收集了四种骨代谢测量和九种蛋白质表达测量。骨代谢与蛋白质之间的关联采用Spearman相关分析和Logistic回归分析。
■我们筛选并验证了3种关键蛋白质,即白细胞介素-11(IL-11),白细胞介素-17(IL-17)和程序性细胞死亡-2(PD-L2)通过微阵列分析与免疫和炎症相关。RA患者IL-2、IL-5、IL-11、IL-17、CTLA4、TNF-β水平高于对照组(P<0.05),同时,RA患者IL-8、PD-L2、TNF-β和B7-2水平均较低(P>0.05)。Spearman相关检验结果提示,夏普得分与年龄呈正相关,CCP与RF呈正相关,SDS评分与RF呈正相关,IL-17与CCP呈正相关,BGP与BALP呈正相关,RANKL与BALP呈正相关,VAS评分与CRP呈负相关,中医评分与SF-36评分呈负相关。
■BALP,BGP,OPG,RANKL与RA患者的免疫炎症相关蛋白和不良SPP密切相关,可用于预测RA患者的不良SPP,尽管潜在的机制需要进一步探索。
UNASSIGNED: In patients with rheumatoid arthritis (RA), osteoarthrosis significantly reduces self-perception. However, the intrinsic relationship between bone metabolism and SPP and cell activity at the molecular level remains unclear. The purpose of this study was to understand the relationship between RA bone metabolic indicators and immune inflammation-related proteins.
UNASSIGNED: A total of 30 patients with RA and 30 healthy controls were recruited. Four bone metabolism measures and nine proteins expression measures were collected from RA patients and healthy controls. Spearman Correlation Analysis and Logistic-regression Analysis were adopted for associations between bone metabolism and proteins.
UNASSIGNED: We screened and verified 3 key proteins, namely interleukin-11 (IL-11), interleukin-17 (IL-17) and programmed cell death-2 (PD-L2) related to immunity and inflammation through microarray analysis. Levels of IL-2, IL-5, IL-11, IL-17, CTLA4, TNF-β were higher in RA patients than in the control group (P<0.05), meanwhile, the levels of IL-8, PD-L2, TNF-β and B7-2 were low in RA patients (P>0.05).The results of Spearman Correlation Test suggested that sharp score was positively correlated with age, CCP was positively correlated with RF, SDS score was positively correlated with RF, IL-17 was positively correlated with CCP, BGP was positively correlated with BALP, RANKL was positively correlated with BALP, VAS score was negatively correlated with CRP, TCM score was negatively correlated with SF-36 score.
UNASSIGNED: BALP, BGP, OPG, RANKL were strongly associated with immune inflammation-related proteins and poor SPP in RA patients, which can be used to predict poor SPP in RA patients, although the underlying mechanisms need to be further explored.