背景:雄激素性脱发(AGA)是一种普遍的脱发障碍,具有心理影响。传统的治疗方法有局限性,导致对再生疗法的探索,例如源自脂肪组织干细胞的外泌体(ASC-Exosomes)。
方法:首先,使用ASC外泌体处理的人毛囊(HF)真皮乳头细胞(hDPC),ALP,VCAN,β-连环蛋白,用RT-PCR和p-GSK3β测定LEF-1水平,GSK3β,β-连环蛋白,ALP,用蛋白质印迹分析评估β-肌动蛋白水平。毛干伸长试验和ALP测定,使用人HF器官培养进行Ki-67和β-catenin。AGA患者接受了ASC-外泌体治疗,并进行了毛发计数评估,摄影评估,主观满意度,和安全概况。
结果:ASC-外泌体影响hDPCs,增加增殖和毛发生长相关基因的上调,包括ALP,VCAN,β-连环蛋白,和LEF-1。Wnt/β-catenin通路被激活,表明它们在促进头发生长方面的作用。ASC-外泌体还促进毛干伸长和ALP活性,暗示了头发再生的潜力。30名AGA参与者登记并治疗超过24周。受试者经历了总头发密度的显着增加,改进全球摄影评估,报告主观满意度较高,无严重不良反应。
结论:这项研究有助于越来越多的证据支持外泌体在脱发治疗中的使用,为AGA患者提供安全有效的替代方案。
BACKGROUND: Androgenetic alopecia (AGA) is a prevalent hair loss disorder with psychological repercussions. Traditional treatments have limitations, leading to the exploration of regenerative therapies such as exosomes derived from adipose tissue stem cells (ASC-Exosomes).
METHODS: First, using human hair follicle (HF) dermal papilla cells (hDPCs) treated with ASC-Exosomes, ALP, VCAN, β-catenin, and LEF-1 levels with RT-PCR and p-GSK3β, GSK3β, β-catenin, ALP, and β-actin levels with western blot analysis were assessed. Hair shaft elongation test and assay for ALP, Ki-67, and β-catenin were done using human HF organ culture. Patients with AGA had ASC-Exosomes treatment and were evaluated for hair counts, photographic assessments, subjective satisfaction, and safety profiles.
RESULTS: ASC-Exosomes impact hDPCs, increasing proliferation and the upregulation of hair growth-related genes, including ALP, VCAN, β-catenin, and LEF-1. The Wnt/β-catenin pathway was activated, indicating their role in promoting hair growth. ASC-Exosomes also promoted hair shaft elongation and ALP activity, suggesting a potential for hair regeneration. Thirty participants with AGA enrolled and treated over 24 weeks. The subjects experienced a significant increase in total hair density, improved global photographic assessments, and reported higher subjective satisfaction without severe adverse reactions.
CONCLUSIONS: This research contributes to the growing body of evidence supporting the use of exosomes in hair loss treatment, offering a safe and effective alternative for individuals with AGA.