Abstract：
Hair loss affects men and women of all ages. Myokines, which are mainly secreted by skeletal muscles during exercise, have numerous health benefits. VEGF, IGF-1, FGF and irisin are reprehensive myokines. Although VEGF, IGF-1 and FGF are positively associated with hair growth, few studies have researched the effects of irisin on hair growth. Here, we investigated whether irisin promotes hair growth using in vitro, ex vivo and in vivo patch assays, as well as mouse models. We show that irisin increases proliferation, alkaline phosphatase (ALP) activity and mitochondrial membrane potential in human dermal papilla cells (hDPCs). Irisin activated the Wnt/β-catenin signalling pathway, thereby upregulating Wnt5a, Wnt10b and LEF-1, which play an important role in hair growth. Moreover, irisin enhanced human hair shaft elongation. In vivo, patch assays revealed that irisin promotes the generation of new hair follicles, accelerates entry into the anagen phase, and significantly increases hair growth in C57BL/6 mice. However, XAV939, a Wnt/β-catenin signalling inhibitor, suppressed the irisin-mediated increase in hair shaft and hair growth. These results indicate that irisin increases hair growth via the Wnt/β-catenin pathway and highlight its therapeutic potential in hair loss treatment.
摘要：
脱发影响所有年龄段的男性和女性。Myokines,主要由骨骼肌在运动时分泌，有许多健康益处。VEGF,IGF-1，FGF和irisin是应受谴责的Myokine。虽然VEGF,IGF-1和FGF与毛发生长呈正相关，很少有研究研究irisin对头发生长的影响。这里,我们研究了irisin是否在体外促进头发生长，离体和体内贴片测定，以及老鼠模型。我们证明irisin增加了增殖，人毛乳头细胞（hDPCs）的碱性磷酸酶（ALP）活性和线粒体膜电位。Irisin激活Wnt/β-catenin信号通路，从而上调WNT5a，Wnt10b和LEF-1在头发生长中起重要作用。此外，irisin增强了人的毛干伸长率。在体内,贴片试验显示irisin促进了新毛囊的生成，加速进入生长期，并显著增加C57BL/6小鼠的毛发生长。然而,XAV939，一种Wnt/β-连环蛋白信号抑制剂，抑制了irisin介导的毛干和头发生长的增加。这些结果表明,irisin通过Wnt/β-连环蛋白途径增加毛发生长,并突出其在脱发治疗中的治疗潜力。
