PDF(Pubmed)
Abstract：
Reptile white blood cell (WBC) morphological features are strikingly variable across species. In the Argentine black and white tegu (Salvator merianae), red tegu (Salvator rufescens), and Savannah monitor (Varanus exanthematicus), previous reports described a WBC type with a single distinct, clear, linear- to ovoid- to crescent-shaped inclusion of presumptive monocytic origin. The objective of this study was to further investigate the origin of this unique WBC type with crescent-shaped inclusions. Blood samples from two Argentine black and white tegus, tegu 1, a 4-year-old female, and tegu 2, a 2-year-old presumed male, were submitted for routine hematological evaluation. Additional blood films were prepared and stained with these cytochemical stains: alkaline phosphatase (ALP; naphthol AS-MX phosphate substrate), alpha-naphthyl butyrate esterase, alpha-chloroacetate esterase, myeloperoxidase, Periodic acid-Schiff, and Sudan black B. Blood films from tegu 1 were also stained with a second ALP stain (5-bromo-4-chloro-3-indoxyl-phosphate and nitroblue tetrazolium substrate), Luna, luxol fast blue, and toluidine blue. The blood from tegu 1 was cytocentrifuged to isolate and fix the buffy coat in glutaraldehyde 2.5% aqueous solution for transmission electron microscopy. Six morphologically distinct WBC types were identified from tegu 1, including heterophils, basophils, monocytes, azurophils, lymphocytes, and the unique WBC type, which were identified as eosinophils with inclusions. WBC types in tegu 2 were similar; however, eosinophils lacked a discernable inclusion. Proper WBC identification will be useful in obtaining accurate hemogram data for this species.
摘要：
爬行动物白细胞（WBC）的形态特征在物种之间差异很大。在阿根廷黑白泰古（Salvatormerianae）中，红色tegu(Salvatorrufescens),和萨凡纳监视器（Varanusexanthematicus），以前的报告描述了一种WBC类型，clear,线性-卵形-新月形包含假定单核细胞起源。这项研究的目的是进一步研究这种具有新月形包裹体的独特WBC类型的起源。阿根廷黑白相间的血样,Tegu1,4岁女性,和Tegu2，一个2岁的男性，进行常规血液学评估。制备额外的血膜并用这些细胞化学染色剂染色:碱性磷酸酶(ALP;萘酚AS-MX磷酸盐底物),α-萘基丁酸酯酶，α-氯乙酸酯酶，髓过氧化物酶,周期性酸-希夫,和苏丹黑B。来自tegu1的血膜也用第二个ALP染色（5-溴-4-氯-3-吲哚基磷酸酯和硝基蓝四唑盐底物）染色，露娜,luxol快蓝,和甲苯胺蓝。将来自tegu1的血液进行细胞离心以分离并固定在2.5％戊二醛水溶液中的血沉棕黄层用于透射电子显微镜。从tegu1中鉴定出六种形态上不同的WBC类型，包括异源性粒细胞，嗜碱性粒细胞,单核细胞,嗜天色粒细胞,淋巴细胞，和独特的WBC类型，被鉴定为嗜酸性粒细胞与夹杂物。tegu2中的白细胞类型相似；然而，嗜酸性粒细胞缺乏可辨别的包涵体。正确的WBC鉴定将有助于获得该物种的准确血象数据。
