目标:在过去的几十年里,全世界对使用植物提取物预防口腔疾病越来越感兴趣。这种兴趣的主要焦点在于鉴定和分离限制微生物生物膜形成的物质,微生物生物膜在龋齿的发展中起着重要作用。牙周炎,和种植体周围炎.在这项体外临床研究中,我们研究了迷迭香提取物对原位初始口腔生物膜中口腔微生物的抗菌作用。
方法:用浓度为20mg/ml和30mg/ml的紫草提取物离体处理来自6名健康志愿者的初始原位生物膜样品(2小时)。通过计数菌落形成单位来确定活细菌细胞的数量。在纯培养物中分离所有存活的细菌,并使用MALDI-TOF和生化测试程序进行鉴定。此外,活/死染色与落射荧光显微镜相结合用于可视化初始生物膜中的抗微生物作用。
结果:经拉索处理的生物膜中菌落形成单位的数量明显低于未经处理的对照(p<0.001)。log10对需氧菌和厌氧菌的还原范围分别为1.64-2.78和2.41-3.23,分别。关于细菌组成,观察到较大的个体内和个体间变异性。除了弯曲杆菌属。,与未处理的生物膜相比,用R.officinalis处理后所有细菌分类群的平均数量较低。在未经处理的生物膜中总共检测到49种不同的物种,而在紫草处理的生物膜中只检测到11种细菌。活的/死的染色证实,与未处理的生物膜相比,经拉丝处理的生物膜具有显著更低数量的存活细菌。
结论:用紫草提取物治疗具有消除微生物口腔初始生物膜的显着潜力。
结论:本研究的结果鼓励在生物膜控制中使用紫杉提取物,从而作为合成物质的草药佐剂治疗龋齿和牙周炎。
OBJECTIVE: In the last few decades, there has been a growing worldwide interest in the use of plant extracts for the prevention of oral diseases. The main focus of this interest lies in the identification and isolation of substances that limit the formation of microbial biofilm which plays a major role in the development of caries, periodontitis, and peri-implantitis. In this clinical ex vivo study, we investigated the antimicrobial effects of Rosmarinus officinalis extract against oral microorganisms within in situ initial oral biofilms.
METHODS: Initial in situ biofilm samples (2 h) from six healthy volunteers were treated ex vivo with R. officinalis extract at concentrations of 20 mg/ml and 30 mg/ml. The number of viable bacterial cells was determined by counting the colony-forming units. All surviving bacteria were isolated in pure cultures and identified using MALDI-TOF and biochemical testing procedures. Additionally, live/dead staining in combination with epifluorescence microscopy was used for visualizing the antimicrobial effects in the initial biofilms.
RESULTS: The number of colony-forming units in the R. officinalis-treated biofilms was significantly lower than in the untreated controls (p < 0.001). The reduction range of log10 was 1.64-2.78 and 2.41-3.23 for aerobic and anaerobic bacteria, respectively. Regarding the bacterial composition, large intra- and interindividual variability were observed. Except for Campylobacter spp., the average amount of all bacterial taxa was lower after treatment with R. officinalis than in the untreated biofilms. A total of 49 different species were detected in the untreated biofilms, while only 11 bacterial species were detected in the R. officinalis-treated biofilms. Live/dead staining confirmed that the R. officinalis-treated biofilms had significantly lower numbers of surviving bacteria than the untreated biofilms.
CONCLUSIONS: The treatment with R. officinalis extract has a significant potential to eliminate microbial oral initial biofilms.
CONCLUSIONS: The results of this study encourage the use of R. officinalis extracts in biofilm control and thus in the treatment of caries and periodontitis as a herbal adjuvant to synthetic substances.