本研究调查了添加对抗疗法阿霉素(DOX0.3µg/ml)的效果,超稀释/动态阿霉素(0.2%乙醇)的载体,超稀释/动态阿霉素的不同动力学(DOX6CH,DOX12CH和DOX30CH),在不存在或存在0.3µg/ml阿霉素诱导的化学应激对卵泡存活和激活的影响下,培养基的抗氧化能力,过氧化氢酶活性(CAT),生产反应蛋白硫醇,维持I型和III型胶原纤维和脂褐素在猪卵巢组织中的积累体外培养48小时。要做到这一点,将部分卵巢组织碎片固定用于未培养的对照,其余的在MEM(培养的对照)中培养,DOX0.3µg/ml,乙醇,DOX6CH,DOX12CH,DOX30CH,DOX(0.3µg/ml)+DOX6CH,DOX(0.3µg/ml)+DOX12CH,DOX(0.3µg/ml)+DOX30CH治疗。结果表明,总的来说,超稀释/动态阿霉素(DOX6CH,DOX12CH和DOX30CH)减轻了同种疗法阿霉素(0.3µg/ml)对腔前卵泡形态的毒性作用,I型和III型胶原纤维的含量,和组织中脂褐素的产生。然而,只有DOX(0.3µg/ml)+DOX6CH减弱了DOX(0.3µg/ml)诱导的氧化应激,保持与未培养对照相似的足够的CAT活性。此外,当考虑三种分离的超稀释/动态阿霉素时,与未培养的对照和MEM相比,只有DOX12CH增加了减少的硫醇水平。总之,用超常规/动态DOX(DOX6CH,DOX12CH和DOX30CH)在封闭在卵巢组织中的猪腔前卵泡的体外培养过程中,减弱了对抗性阿霉素诱导的毒性。
The present study investigated the effect of adding allopathic doxorubicin (DOX 0.3 µg/mL), the vehicle of ultradiluted/dynamized doxorubicin (0.2 % ethanol), different dynamizations of ultradiluted/dynamized doxorubicin (DOX 6CH, DOX 12CH and DOX 30CH), both in the absence or presence of chemical stress induced by doxorubicin at 0.3 µg/mL on follicular survival and activation, antioxidant capacity of the medium, Catalase activity (CAT), production of reactive protein thiol, maintenance of type I and III collagen fibers and accumulation of
lipofuscin in porcine ovarian tissue cultured in vitro for 48 hours. To do this, part of the ovarian tissue fragments was fixed for the uncultured control and the rest were cultured in: MEM (cultured control), DOX 0.3 µg/mL, Ethanol, DOX 6CH, DOX 12CH, DOX 30CH, DOX (0.3 µg/mL) + DOX 6CH, DOX (0.3 µg/mL) + DOX 12CH, DOX (0.3 µg/mL) + DOX 30CH treatments. The results showed that, in general, ultradiluted/dynamized doxorubicin (DOX 6CH, DOX 12CH and DOX 30CH) mitigated the toxic effect of allopathic doxorubicin (0.3 µg/mL) on the morphology of preantral follicles, the content of type I and III collagen fibers, and the production of
lipofuscin in the tissue. However, only DOX (0.3 µg/mL) + DOX 6CH attenuated the oxidative stress induced by DOX (0.3 µg/mL), maintaining adequate CAT activity that was similar to the uncultured control. Additionally, when the three isolated ultradiluted/dynamized doxorubicin were considered, only DOX 12CH increased the reduced thiol levels compared to the uncultured control and MEM. In conclusion, supplementing the culture medium with ultradiluted/dynamized DOX (DOX 6CH, DOX 12CH and DOX 30CH) attenuated the toxicity induced by allopathic doxorubicin during the in vitro culture of pig preantral follicles enclosed in ovarian tissue.