Abstract：
The very-low calorie ketogenic diet (VLCKD) is recommended as an effective dietary approach for the management of obesity. This study investigated changes in circulating biomarkers of redox homeostasis induced by a multiphase VLCKD in obese individuals. A total of 40 obese subjects were prescribed a multiphasic VLCKD for eleven weeks. Anthropometric measurements, body composition parameters, calorimetric measures, and standard laboratory markers of glucose and lipid metabolism were evaluated at baseline (T0) and at the end of the dietary intervention (T1). Additionally, circulating markers of oxidative damage and antioxidant status were analyzed in serum and erythrocytes. Compared to T0, at T1 the multiphase VLCKD induced significant weight loss and reduction of waist circumference, with beneficial effects on body composition parameters and the glucose/lipid biochemical profile. Moreover, a decrease in serum markers of oxidative damage was reported at T1, while no changes in serum markers of antioxidant status and in erythrocyte redox markers were observed. In addition, a significant association was found between variations in anthropometric measurements, body composition, glucose metabolism parameters, and changes in circulating markers of oxidative damage. Regression models showed that variation in lipofuscin was significant predictor of changes in body mass index, fat mass, visceral adiposity, and insulin sensitivity. In conclusion, this study demonstrated that the multiphase VLCKD improves serum redox balance by reducing markers of oxidative damage in obese individuals, highlighting the interplay between adiposity, glucose metabolism, and redox homeostasis in the pathogenesis of obesity. Furthermore, these data provide a rationale for future investigations aimed at testing serum lipofuscin as a reliable redox marker in obesity.
摘要：
建议将极低热量生酮饮食（VLCKD）作为控制肥胖的有效饮食方法。这项研究调查了肥胖个体中多相VLCKD诱导的氧化还原稳态的循环生物标志物的变化。总共40名肥胖受试者接受了11周的多相VLCKD治疗。人体测量,身体成分参数，量热措施，在基线(T0)和饮食干预结束时(T1)评估糖脂代谢的标准实验室标志物.此外,在血清和红细胞中分析了氧化损伤和抗氧化状态的循环标志物。与T0相比，在T1时，多相VLCKD引起明显的体重减轻和腰围减少，对身体成分参数和葡萄糖/脂质生化特征具有有益的影响。此外，血清氧化损伤标志物在T1时降低,而血清抗氧化状态标志物和红细胞氧化还原标志物没有变化.此外,在人体测量的变化之间发现了显着的关联，身体成分,葡萄糖代谢参数，以及氧化损伤循环标志物的变化。回归模型显示，脂褐素的变化是体重指数变化的重要预测因子，脂肪量,内脏肥胖,和胰岛素敏感性。总之,这项研究表明，多相VLCKD通过减少肥胖个体的氧化损伤标志物来改善血清氧化还原平衡，强调肥胖之间的相互作用，葡萄糖代谢，和氧化还原稳态在肥胖发病机制中的作用。此外，这些数据为未来旨在检测血清脂褐素作为肥胖患者可靠的氧化还原标志物的研究提供了理论基础.
