根据以前的家猫临床经验(Feliscatus),评估非家庭猫科动物的ABC血型和血液(内)相容性的能力,并充分考虑和计划输血,可能很重要。尽管非家养猫科动物似乎具有与家猫相似的ABC血型系统,尚未报道用即时护理试剂盒和通过CMAH基因分型对家猫进行分型。在这项研究中,来自18种不同非家养动物物种的162份血液样本(猎豹[Acinonyxjubatus,n=42],狮子[PantheraLeo,n=33],老虎[提格里斯,n=23],加拿大山猫[加拿大山猫,n=11],雪豹[Unciauncia,n=10],puma[Pumaconcolor,n=7],云豹[Neofelisnebulosa,n=6],随从[Leptailurus随从,n=5],美洲虎[Pantheraonca,n=5],钓鱼猫[Prionailurusviverrinus,n=4],帕拉斯猫[·费利斯·曼努尔,n=3],山猫[山猫鲁弗斯,n=3],ocelot[Leoparduspardalis,n=3],黑脚猫[Felisnigripes,n=2],豹子[PantheraPardus,n=2],非洲野猫[Felislybica,n=1],卡拉卡尔[卡拉卡尔,n=1],和沙猫[·费里斯·玛格丽塔,n=1])是通过实验室和护理点测试对ABC血液进行分型的,对B型和C型(AB)表型的四种已知CMAH变体进行基因分型,并与彼此和家养的A型猫交叉匹配。传统的试管分型确定A型血(n=106),B型(n=8),C型(n=43),并且没有可辨别的ABC类型(n=4)。在护理点和传统的打字测试结果之间发现了一些差异。在家猫中,测试的猫科动物都不具有负责B型和C型(AB)的四种CMAH变体。在非家养猫科动物物种内部和之间以及ABC不相容性之间确定了交叉配型不相容性(≥2凝集)。在家猫和各种非家猫之间进行的26次交叉比赛中,只有7人(27%)是相容的。总之,即时分型试剂盒和CMAH基因分型,成功用于家猫,可能无法识别非家庭猫的正确ABC血型。建议事先进行交叉匹配,以增加任何非家庭动物之间兼容输血的可能性。
Based upon previous clinical experience with domestic cats (Felis catus), the ability to assess ABC blood types and blood (in-)compatibilities of nondomestic felids, and adequately consider and plan for blood transfusions, may be important. Although nondomestic felids appear to have an ABC blood group system similar to domestic cats, typing with point-of-care kits and by CMAH genotyping for domestic cats have not been reported. In this study, 162 blood samples from 18 different nondomestic felid species (cheetah [Acinonyx jubatus, n = 42], lion [Panthera leo, n = 33], tiger [Panthera tigris, n = 23], Canada lynx [Lynx canadensis, n = 11], snow leopard [Uncia uncia, n = 10], puma [Puma concolor, n = 7], clouded leopard [Neofelis nebulosa, n = 6], serval [Leptailurus serval, n = 5], jaguar [Panthera onca, n = 5], fishing cat [Prionailurus viverrinus, n = 4], Pallas cat [Felis manul, n = 3], bobcat [Lynx rufus, n = 3], ocelot [Leopardus pardalis, n = 3], black footed cat [Felis nigripes, n = 2], leopard [Panthera pardus, n = 2], African wildcat [Felis lybica, n = 1], caracal [Caracal caracal, n = 1], and sand cat [Felis margarita, n = 1]) were ABC blood typed by laboratory and point-of-care tests, genotyped for four known CMAH variants for type B and type C (AB) phenotypes, and crossmatched with one another and domestic type A cats. Traditional tube typing identified blood type A (n = 106), type B (n = 8), type C (n = 43), and no discernible ABC type (n = 4). Several discrepancies were found between point-of-care and traditional typing test results. None of the tested felids possessed the four CMAH variants responsible for type B and C (AB) in domestic cats. Crossmatch incompatibilities (≥2+ agglutination) were identified within and between nondomestic felid species and beyond ABC incompatibilities. Of 26 crossmatches performed between domestic cats and various nondomestic felids, only 7 (27%) were compatible. In conclusion, point-of-care typing kits and CMAH genotyping, successfully used in domestic cats, may not identify the correct ABC blood type in nondomestic felids. Prior crossmatching is recommended to increase the likelihood of compatible transfusions between any nondomestic felids.