光,作为一个关键的环境因素,在光合作用中起着举足轻重的作用,最终影响茶树芽冲洗的时机。然而,不同光周期和光质对白化茶品种“黄葵”(发芽后期品种)芽冲洗时间的协同作用尚不清楚。因此,本研究的目的是研究不同光周期(12L/12D,14L/10D,16L/8D,和18L/6D,其中L=日光小时数,D=黑暗小时数)和红色(R)与蓝色(B)光的比率(R/B1:1,R/B1:2,R/B1:3和R/B2:1)对白化茶品种“黄葵”的发芽和生长。在我们的研究中,我们通过调查芽冲洗的时间,研究了不同的光周期和红光和蓝光如何影响茶的发芽和生长,光合作用,叶绿素含量,和增长指标。首先,我们的研究表明,“黄葵”发芽4天,2天,与在12L/12D下栽培的植物相比,在一芽一叶期的16L/8D光周期下提前了1天,14L/10D,和18L/6D光周期在光下模拟太阳光谱。此外,在16L/8D光周期处理下,“黄葵”的生长得到了最大的促进。此外,与其他处理相比,在R/B2:1(红色/蓝色)处理下,16L/8D光周期观察到“黄葵”的最早发芽。此外,最大的植物高度,新芽的长度,在R/B2:1的白化茶品种“黄葵”中检测到新的叶面积。此外,生长素的含量(吲哚乙酸,R/B2:1下的IAA)和反式玉米素(tZ)显着高于R/B1:1和16L/8D光周期的对照处理。此外,在R/B2:1处理下,CsIAA13,CsGH3.1,CsAUX1和CsARF2的生长素相关表达水平显着高于对照组。CsARR-B的表达,细胞分裂素相关基因的正调节因子,在R/B2:1治疗下明显高于对照治疗下,而负调节因子CsARR-A的表达则相反。因此,用16L/8D光周期的R/B2:1处理是白化茶品种“黄葵”的芽冲洗时间的适当方法,这可能与IAA或tZ信号转导有关。总之,我们的研究提供了一种新颖的照明策略,可以促进白化茶品种的发芽和生长。
Light, as a critical environmental factor, plays a pivotal role in photosynthesis, ultimately influencing the timing of bud flush in tea plants. However, the synergistic effects of different photoperiods and light qualities on the timing of bud flush in the albino tea cultivar \'HuangKui\' (later germination variety) remain unknown. Thus, the objective of this study was to investigate the effects of different photoperiods (12L/12D, 14L/10D, 16L/8D, and 18L/6D, where L = the number of daylight hours and D = the number of hours of darkness) and ratios of red (R) to blue (B) light (R/B 1:1, R/B 1:2, R/B 1:3, and R/B 2:1) on the germination and growth of the albino tea variety \'HuangKui\'. In our study, we examined how different photoperiods and red light and blue light affected tea germination and growth by investigating the timing of bud flush, photosynthesis, chlorophyll content, and growth indicators. First, our study showed that \'HuangKui\' germinated 4 days, 2 days, and 1 day earlier under the 16L/8D photoperiod at the one bud and one leaf period compared with plants cultivated under the 12L/12D, 14L/10D, and 18L/6D photoperiods under light simulating the solar spectrum. Also, the growth of \'HuangKui\' was maximumly promoted under the 16L/8D photoperiod treatment. Additionally, the earliest germination of \'HuangKui\' was observed for the 16L/8D photoperiod under the R/B 2:1 (red/blue) treatment compared with the other treatments. Moreover, the greatest plant height, length of the new shoots, and new leaf areas were detected in the albino tea variety \'HuangKui\' under R/B 2:1. Moreover, the contents of auxin (indole acetic acid, IAA) and trans-zeatin (tZ) under R/B 2:1 were significantly higher than those under the R/B 1:1 and control treatments with the 16L/8D photoperiod. Additionally, the auxin-related expression levels of CsIAA13, CsGH3.1, CsAUX1, and CsARF2 under the R/B 2:1 treatment were significantly higher than those in the control. The expression of CsARR-B, a positive regulator of cytokinin-related genes, was significantly higher under the R/B 2:1 treatment than under the control treatment, while the opposite result was found for the expression of the negative regulator CsARR-A. Therefore, the R/B 2:1 treatment with the 16L/8D photoperiod was an appropriate means of timing the bud flush for the albino tea variety \'HuangKui\', which may be related to IAA or tZ signal transduction. In conclusion, our research offers a novel lighting strategy that promotes the germination and growth of albino tea cultivars.