背景:芬太尼试纸(FTS)是药物检查中常用的工具,用于在食用前测试街头药物样本中是否存在芬太尼。以前的报道表明,除了芬太尼,FTS还可以检测芬太尼类似物,如乙酰芬太尼和丁酰芬太尼,关于它们检测芬太尼类似物如卡芬太尼和呋喃基芬太尼的能力的报道相互矛盾。然而数百种已知的芬太尼类似物,目前还没有一项大规模的研究来合理化FTS对不同芬太尼类似物的反应性.
方法:在本研究中,在两种品牌的芬太尼试纸上筛选了251种合成阿片类药物,包括214种芬太尼类似物,以(1)评估两种品牌的芬太尼试纸检测芬太尼相关化合物的能力差异,以及(2)确定芬太尼类似物化学结构中的哪些部分对于FTS检测最关键。在这项研究中评估了两个FTS品牌:BTNX快速反应和WHPMDanceSafe。
结果:在评估的251个筛选化合物中,通过BTNX和DanceSafeFTS可检测到121种化合物的浓度为或低于20,000ng/mL,任何一个品牌都检测不到50个,一个品牌检测到80个,但另一个品牌检测不到(n=52个BTNX,n=28DanceSafe)。筛选的芬太尼类似物的结构分析显示,一般来说,对苯乙基部分的大量修饰抑制BTNXFTS的检测,而对羰基部分的大量修饰抑制DanceSafeFTS的检测。
结论:不同的“盲点”是由用于引发这些不同条带的抗体的不同半抗原引起的。通过在常规药物检查中利用两个品牌的FTS,用户可以增加在一个品牌的“盲点”中检测芬太尼类似物的机会。
Fentanyl test strips (FTS) are a commonly deployed tool in drug checking, used to test for the presence of fentanyl in street drug samples prior to consumption. Previous reports indicate that in addition to fentanyl, FTS can also detect fentanyl analogs like acetyl fentanyl and butyryl fentanyl, with conflicting reports on their ability to detect fentanyl analogs like Carfentanil and furanyl fentanyl. Yet with hundreds of known fentanyl analogs, there has been no large-scale study rationalizing FTS reactivity to different fentanyl analogs.
In this study, 251 synthetic opioids-including 214 fentanyl analogs-were screened on two brands of fentanyl test strips to (1) assess the differences in the ability of two brands of fentanyl test strips to detect fentanyl-related compounds and (2) determine which moieties in fentanyl analog chemical structures are most crucial for FTS detection. Two FTS brands were assessed in this study: BTNX Rapid Response and WHPM DanceSafe.
Of 251 screened compounds assessed, 121 compounds were detectable at or below 20,000 ng/mL by both BTNX and DanceSafe FTS, 50 were not detectable by either brand, and 80 were detectable by one brand but not the other (n = 52 BTNX, n = 28 DanceSafe). A structural analysis of fentanyl analogs screened revealed that in general, bulky modifications to the phenethyl moiety inhibit detection by BTNX FTS while bulky modifications to the carbonyl moiety inhibit detection by DanceSafe FTS.
The different \"blind spots\" are caused by different haptens used to elicit the antibodies for these different strips. By utilizing both brands of FTS in routine drug checking, users could increase the chances of detecting fentanyl analogs in the \"blind spot\" of one brand.