Abstract:
Bisphenol S (BPS) is a common pollutant in the environment and has posed a potential threat to aquatic animals and human health. To accurately assess the pollution level and ecological risk of BPS, there is an urgent need to establish simple and sensitive detection methods for BPS. In this study, BPS complete antigen was successfully prepared by introducing methyl 4-bromobutyrate and coupling bovine serum albumin (BSA). The monoclonal antibody against BPS (anti-BPS mAb) with high affinity (1: 256,000) was developed based on the BPS complete antigen, which showed low cross-reactivity with BPS structural analogues. Then, an electrochemical immunosensor was constructed to detect BPS using multi-walled carbon nanotubes and gold nanoflower composites as signal amplification elements and using anti-BPS mAb as the probe. The electrochemical immunosensor had a linear range from 1 to 250 ng⋅mL-1 and a limit of detection (LOD) down to 0.6 ng⋅mL-1. Additionally, a more stable and sensitive lateral flow immunoassay (LFIA) for BPS was developed based on iridium oxide nanoparticles, with a visual detection limit of 1 ng⋅mL-1, which was 10 times lower than that of classical Au-NPs LFIA. After evaluation of their stability and specificity, the reliability of these two methods were further validated by measuring BPS concentrations in the water and fish tissues. Thus, this study provides sensitive, robust and rapid methods for the detection of BPS in the environment and organisms, which can provide a methodological reference for monitoring environmental contaminants.
摘要:
双酚S(BPS)是环境中常见的污染物,对水生动物和人类健康构成潜在威胁。为了准确评估BPS的污染水平和生态风险,迫切需要建立简便、灵敏的BPS检测方法。在这项研究中,通过引入4-溴丁酸甲酯和偶联牛血清白蛋白(BSA)成功制备了BPS完全抗原。以BPS完全抗原为基础,研制出高亲和力(1:256000)的抗BPS单克隆抗体,显示与BPS结构类似物的低交叉反应性。然后,以多壁碳纳米管和金纳米花复合材料为信号放大元件,以抗BPSmAb为探针,构建了电化学免疫传感器来检测BPS。电化学免疫传感器的线性范围为1至250ng·mL-1,检测限(LOD)低至0.6ng·mL-1。此外,基于氧化铱纳米颗粒,开发了一种更稳定、更灵敏的BPS侧流免疫分析法(LFIA),视觉检测限为1ng·mL-1,比经典Au-NPLFIA低10倍。在评估其稳定性和特异性后,通过测量水和鱼组织中的BPS浓度进一步验证了这两种方法的可靠性。因此,这项研究提供了敏感的,在环境和生物中检测BPS的稳健和快速的方法,为环境污染物监测提供方法参考。