在开花植物中,LEAFY(LFY)基因控制花分生组织的活性。在早期的陆地植物中,如苔藓和蕨类植物,it,然而,在二倍体孢子体的细胞分裂和发育中作用最小。同源性建模,一种准确有效的蛋白质结构预测方法,用于构建非开花和开花植物中LEAFY蛋白的3D模型。本研究检查了以下物种:植物绿藻,Physcomitrella,Ceratopteris,Picea,和拟南芥,因为它们是发育研究中普遍使用的模式生物。来自模型生物的LEAFY蛋白序列通过多序列比对进行比对。使用PHYRE2程序以100%置信度构建来自所有模型生物的LEAFY蛋白的3D模型,并使用MolProbity工具对构建的模型进行评估。根据保守地区,绿藻绿藻与Physcomitrellasp具有38-46%的序列相似性。,与Ceratopterissp.37-46%的相似性。,与云杉属33-41%的相似性。,与拟南芥的相似性为32-38%。MotifFinder服务器确定了蛋白质家族域FLO_LFY和LFY_SAM,其功能是花分生组织发育。二级结构预测分析表明,LEAFY蛋白属于α(α)蛋白类,它对突变稳定,因此限制了LEAFY蛋白的结构变化。研究发现揭示了来自共同祖先绿藻的LFY基因的两个不同簇。一簇存在于非开花植物中,包括苔藓,蕨类植物,和裸子植物,另一簇存在于开花植物中,包括兰花,单子叶植物,双子叶,和被子植物.
In flowering plants, the LEAFY (
LFY) gene controls floral meristem activity. In early land plants such as mosses and ferns, it, however, has a minimum role in cell division and development of diploid sporophyte. Homology modeling, an accurate and efficient protein structure prediction method, was used to construct a 3D model of the LEAFY protein in nonflowering and flowering plants. The present study examines the following species: Charophyte green algae, Physcomitrella, Ceratopteris, Picea, and Arabidopsis, as they are the popularly used model organisms for developmental studies. LEAFY protein sequences from the model organisms were aligned by multiple sequence alignment. 3D models of the LEAFY protein from all the model organisms was constructed using the PHYRE2 program with 100% confidence, and the constructed models were evaluated using the MolProbity tool. On the basis of the conserved regions, Charophyte green algae shared 38-46% sequence similarity with Physcomitrella sp., 37-46% similarity with Ceratopteris sp., 33-41% similarity with Picea sp., and 32-38% similarity with Arabidopsis sp. The Motif Finder server identified the protein family domain FLO_LFY and LFY_SAM, whose function is floral meristem development. Secondary structure prediction analysis indicated that the LEAFY protein belongs to the alpha (α) protein class, which is stable against mutation and thus limits structural changes in the LEAFY protein. The study findings reveal two distinct clusters of the
LFY gene from the common ancestor green algae. One cluster is present in nonflowering plants that include mosses, pteridophytes, and gymnosperms, and the other cluster is present in flowering plants that include orchids, monocots, dicots, and angiosperms.