PDF(Pubmed)
Abstract：
Flowering is a crucial stage for plant reproductive success; therefore, the regulation of plant flowering has been widely researched. Although multiple well-defined endogenous and exogenous flowering regulators have been reported, new ones are constantly being discovered. Here, we confirm that a novel plant growth regulator guvermectin (GV) induces early flowering in Arabidopsis. Interestingly, our genetic experiments newly demonstrated that WRKY41 and its homolog WRKY53 were involved in GV-accelerated flowering as positive flowering regulators. Overexpression of WRKY41 or WRKY53 resulted in an early flowering phenotype compared to the wild type (WT). In contrast, the w41/w53 double mutants showed a delay in GV-accelerated flowering. Gene expression analysis showed that flowering regulatory genes SOC1 and LFY were upregulated in GV-treated WT, 35S:WRKY41, and 35S:WRKY53 plants, but both declined in w41/w53 mutants with or without GV treatment. Meanwhile, biochemical assays confirmed that SOC1 and LFY were both direct targets of WRKY41 and WRKY53. Furthermore, the early flowering phenotype of 35S:WRKY41 lines was abolished in the soc1 or lfy background. Together, our results suggest that GV plays a function in promoting flowering, which was co-mediated by WRKY41 and WRKY53 acting as new flowering regulators by directly activating the transcription of SOC1 and LFY in Arabidopsis.
摘要：
开花是植物繁殖成功的关键阶段；因此，植物开花的调控已被广泛研究。尽管已经报道了多种明确定义的内源性和外源性开花调节剂，新的不断被发现。这里,我们证实了一种新型的植物生长调节剂古维菌素（GV）诱导拟南芥的早期开花。有趣的是,我们的遗传实验最新证明，WRKY41及其同源物WRKY53参与了GV加速开花，作为正开花调节剂。与野生型(WT)相比,WRKY41或WRKY53的过表达导致早期开花表型。相比之下,w41/w53双突变体显示GV加速开花延迟。基因表达分析表明，开花调节基因SOC1和LFY在GV处理的WT中上调，35S：WRKY41和35S：WRKY53植物，但是在有或没有GV处理的w41/w53突变体中两者均下降。同时,生化试验证实SOC1和LFY都是WRKY41和WRKY53的直接靶标。此外，35S：WRKY41品系的早期开花表型在soc1或lfy背景中被废除。一起,我们的结果表明，GV在促进开花中起作用，WRKY41和WRKY53通过直接激活拟南芥中SOC1和LFY的转录而作为新的开花调节因子共同介导。
