背景:尽管在国外研究中已证明载脂蛋白(apo)CIII基因多态性与血脂水平升高有关,不同人群的结果并不总是一致的.此外,这项研究的重点是apoCIII对汉族受试者血脂水平的影响,这在以前没有进行过。
目的:探讨apoCIII基因C3175G和T3206G多态性与血脂水平及其他高脂血症危险因素的关系。汉族男性。
方法:从重庆市两家医院随机抽取健康男性体检者337例,中国。通过DNA测序,鉴定了apoCIII基因C3175G和T3206G多态性,并进一步分析了它们与血脂水平的关系。
结果:apoCIII(3175)GG基因型的携带者的TG水平高于其他基因型(P<0.05)。分层选择三酰甘油(TG)后,在相对较高的TG组中,apoCIIIT3206G的G基因位点与降低总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)的含量有关,而apoCIIIC3175G的G基因位点具有相反的作用。TG非条件Logistic回归结果表明,apoCIIIT3206G多态性的G基因位点也有利于降低TG。
结论:在apoCIII(3175)GG基因型携带者中检测TG是汉族男性高甘油三酯血症的有效预测指标。apoCIII(3206)的G基因位点可能有利于降低血清脂质水平。
BACKGROUND: Although apolipoprotein (apo) CIII gene polymorphisms have proved to be related to the increased serum lipid level in foreign studies, the results have not always been consistent among diverse populations. In addition, the research focuses on the impact of the apoCIII on the serum lipid levels of Han Chinese subjects which was not conducted before.
OBJECTIVE: To explore the relationship between the apoCIII gene C3175G and T3206G polymorphisms and serum lipid levels as well as other risk factors for hyperlipidaemia, in Han Chinese males.
METHODS: A total of 337 healthy male participants undergoing physical examinations were randomly selected from two hospitals in Chongqing, China. Through DNA sequencing, apoCIII gene C3175G and T3206G polymorphisms were identified and their relationships with serum lipid levels were further analysed.
RESULTS: Carriers of apoCIII(3175) GG genotypes have higher levels of TG than other genotypes (P < 0.05). After the stratified selection of triacylglycerol (TG), G gene loci of apoCIIIT3206G are associated with decreasing the content of total cholesterol (TC) and low density lipoprotein-cholesterol (LDL-C) in relatively high TG group while the G gene loci of apoCIIIC3175G have an inverse effect. The outcome of TG unconditional logistic regression shows that the G gene loci of apoCIIIT3206G polymorphism are also beneficial for decreasing TG.
CONCLUSIONS: The detection of TG in apoCIII(3175) GG genotype carriers is an efficient predictor of hypertriglyceridaemia in Han Chinese males. The G gene loci of apoCIII(3206) may be beneficial for decreasing serum lipid level.