背景本研究旨在评估载脂蛋白E(APOE)基因多态性与血清脂质和炎症标志物的关系,以确定其在预测心血管疾病(CVD)和阿尔茨海默病(AD)风险中的潜在作用。方法在VibrantAmerica临床实验室中,共有915名个体接受了脂质和炎症生物标志物的测试。临床数据,血脂和炎症特征,使用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析APOE基因分型。结果与E3/E3基因型相比,具有E2/E3基因型的个体显示出更高水平的高密度脂蛋白(HDL),甘油三酯,载脂蛋白A(APOA),高敏C反应蛋白(hs-CRP),和髓过氧化物酶(MPO)。E2/E4基因型携带者HDL水平较高,甘油三酯,Lp(a),和N末端B型利钠肽原(BNPNT)。E3/E4基因型与总胆固醇水平升高有关,LDL,Lp(a),hs-CRP,低密度脂蛋白(SDLDL),氧化LDL(OXLDL),MPO,LDL-CAL,PLAC,和APOB。E4/E4组显示出更高的总胆固醇浓度,LDL,APOB,Lp(a),hs-CRP,SDLDL,OXLDL,MPO,LDLCAL,和PLAC相比E3/E3载波。这些发现突出了ε4等位基因的潜在致动脉粥样硬化作用和ε2等位基因基于脂质和炎症标志物谱的保护作用。结论本研究提供了将APOE基因多态性与血脂和炎症异常联系起来的有力证据。携带ε4等位基因的个体表现出异常的脂质代谢和异常的炎症标志物,增加他们患CVD和AD的风险。早期发现和及时诊断对于实施治疗至关重要,饮食,和生活方式干预,以减轻风险,预防或延缓脂质和炎症相关疾病。
Background The study aims to assess the association of apolipoprotein E (APOE) gene polymorphisms with serological lipid and inflammatory markers to determine their potential role in predicting the risk of cardiovascular diseases (CVDs) and Alzheimer\'s disease (AD). Methodology A total of 915 individuals underwent testing for lipid and inflammatory biomarkers at Vibrant America Clinical Laboratory. Clinical data, blood lipid and inflammatory profiles, and APOE genotyping were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results Compared to the E3/E3 genotype, individuals with E2/E3 genotypes showed higher levels of high-density lipoprotein (HDL), triglycerides, apolipoprotein A (APOA), high-sensitivity C-reactive protein (hs-CRP), and myeloperoxidase (MPO). E2/E4 genotype carriers had higher levels of HDL, triglycerides, Lp(a), and N-terminal pro b-type natriuretic peptide (BNPNT). E3/E4 genotypes were associated with elevated levels of total cholesterol,
LDL, Lp(a), hs-CRP, small-density low-density lipoprotein (SDLDL), oxidized
LDL (OXLDL), MPO,
LDL-CAL, PLAC, and APOB. The E4/E4 group displayed higher concentrations of total cholesterol,
LDL, APOB, Lp(a), hs-CRP, SDLDL, OXLDL, MPO, LDLCAL, and PLAC compared to E3/E3 carriers. These findings highlight the potential atherogenic effect of the ε4 allele and the protective effect of the ε2 allele based on lipid and inflammatory marker profiles. Conclusions This study provides strong evidence linking APOE gene polymorphism to abnormal serum lipid and inflammatory profiles. Individuals carrying the ε4 alleles exhibited dysregulated lipid metabolism and abnormal inflammatory markers, increasing their risk of CVD and AD. Early detection and prompt diagnosis are crucial for implementing therapeutic, dietary, and lifestyle interventions to mitigate risks and prevent or delay lipid and inflammation-related disorders.