本文提供的数据支持在将其注射到液相色谱-串联质谱(LC-MS/MS)系统中以监测肾上腺素水平之前的大鼠脑样品制备程序,去甲肾上腺素,谷氨酸,γ-氨基丁酸,多巴胺,5-羟色胺,5-羟基吲哚乙酸,和3-甲氧基-4-羟基苯基二醇。此外,我们描述了方法验证测定(如校准曲线,定量下限,日间和日间的精度和准确性,选择性,提取回收率和基质效应,稳定性,和结转效果)根据美国食品和药物管理局和欧洲药品管理局的要求,一步测量不同的神经递质及其代谢物。本文提供的数据与题为“使用液相色谱串联质谱同时测定大鼠脑中8种神经递质及其代谢物水平:在小鼠抑郁症Nrf2模型中的应用”的研究有关(Wojnicz等人。2016)[1]。
The data presented in this article supports the rat brain sample preparation procedure previous to its injection into the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system to monitor levels of adrenaline, noradrenaline, glutamic acid, γ-aminobutyric acid, dopamine, 5-hydroxytryptamine, 5-hydroxyindole acetic acid, and 3-methoxy-4-hydroxyphenylglycol. In addition, we describe the method validation assays (such as calibration curve, lower limit of quantification, precision and accuracy intra- and inter-day, selectivity, extraction recovery and matrix effect, stability, and carry-over effect) according to the United States Food and Drug Administration and European Medicine Agency to measure in one step different neurotransmitters and their metabolites. The data supplied in this article is related to the research study entitled: \"Simultaneous determination of 8 neurotransmitters and their metabolite levels in rat brain using liquid chromatography in tandem with mass spectrometry: application to the murine Nrf2 model of depression\" (Wojnicz et al. 2016) [1].