目的:本研究是对人工育种研究中心饲养的15只KaranFries(KF)公牛的精液射精进行的,国家乳品研究所,Karnal,印度的目标是评估公牛的常规和基于荧光的精液质量分析之间的关系。
方法:从15只KF(荷斯坦弗里斯[HF]杂种)公牛中收集了96只射精。对精液进行颜色评估,volume,质量活动(MA)和个体运动性百分比(IM),精子浓度,活精子的百分比,低渗肿胀试验和顶体完整性,染色质完整性,精子活力,和膜的完整性。数据采用SPSS软件包进行描述性分析。通过Spearman的等级相关系数计算了基于常规和荧光精液参数的父亲等级之间的相关性。
结果:平均射精量(ml),精子浓度(10(6)/ml),MA,IM(%),live(%),形态学异常(%),主机(%),顶体完整性(%),色霉素A3(CMA3)(%),SYBR-PI(%),荧光异硫氰酸盐-花生凝集素(FITC-PNA)(%)分别为4.57±0.36、1162.98±97.93、2.95±0.09、60.8±1.22、71.41±2.10、9.31±1.15、65.5±1.81、86.6±1.59、3.53±0.43、65.39±2.23和74.47±2.53。发现SYBR-PI和FITC-PNA的等级相关性是显着的,大多数参数都是通过常规方法评估的。总的来说,在常规标准中,IM显示公牛的排名几乎与荧光标准相似。
结论:我们的结果概述表明,在常规标准中,MA和IM显示公牛的排名几乎与荧光标准相似。
OBJECTIVE: The present study was carried out on semen ejaculates of 15 Karan Fries (KF) bulls maintained at Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, India with an objective to evaluate the relationship between the conventional and fluorescent based semen quality analysis of the bulls.
METHODS: A total of 96 ejaculates were collected from 15 KF (Holstein Friesian [HF] crossbred) bulls. Semen were evaluated for color, volume, mass activity (MA) and percentage of individual motility (IM), sperm concentration, percent live spermatozoa, hypo-osmotic swelling test and acrosome integrity, chromatin integrity, sperm viability, and membrane integrity. Data were analyzed using SPSS software package for descriptive analysis. The correlation between rankings of sires based on conventional and fluorescent semen parameters were calculated by Spearman\'s rank correlation coefficient.
RESULTS: The average ejaculates volume (ml), sperm concentration (10(6)/ml), MA, IM (%), live (%), morphological abnormalities (%), host (%), acrosome integrity (%), chromomycin A3 (CMA3) (%), SYBR-PI (%), and fluorescent isothiocyanate-peanut agglutinin (FITC-PNA) (%) were 4.57±0.36, 1162.98±97.93, 2.95±0.09, 60.8±1.22, 71.41±2.10, 9.31±1.15, 65.5±1.81, 86.6±1.59, 3.53±0.43, 65.39±2.23 and 74.47±2.53, respectively. Rank correlations were found to be significant for SYBR-PI and FITC-PNA with most of the parameters evaluated by conventional methods. Overall, among conventional criteria, IM revealed ranking of bulls almost similar to that of fluorescent criteria.
CONCLUSIONS: Overview of our results indicated that, among conventional criteria, MA and IM revealed ranking of bulls almost similar to that of fluorescent criteria.