背景:类杆菌属代表了在海洋生态系统中发现的异养细菌的很大比例。类杆菌门的成员积极参与生物聚合物如多糖和蛋白质的降解。拟杆菌基因组表现出各种酶的显著富集,包括碳水化合物活性酶(CAZymes),羧肽酶,酯酶,异构酶,肽酶,磷酸酶,和硫酸酯酶.马里维加属,拟杆菌属中的Marivirgaceae家族的成员,包括六个有记录的物种。在微生物多样性研究中,三种新的Marivirga菌株(BKB1-2T,ABR2-2和BDSF4-3T)从西海分离,大韩民国。
结果:为了探索新分离株的分类状态和基因组特征,我们采用了多相分类方法,其中包括系统发育,化学分类学和综合基因组分析。三个分离株为革兰氏染色阴性,有氧,杆状,中度嗜盐,有滑行运动。两个分离株之间的平均核苷酸同一性(ANI)和数字DNA-DNA杂交(dDDH)值,BKB1-2T和BDSF4-3T,六个参考菌株ANI为70.5-76.5%,dDDH为18.1-25.7%。有趣的是,京都基因和基因组百科全书(KEGG)分析表明,这些菌株具有将硝酸盐异化还原为铵(DNRA)的综合途径的基因,以及其他还原亚硝酸盐的氮途径,一氧化氮,和一氧化二氮.此外,抗SMASH分析表明,该菌株包含3至8个与次生代谢产物合成相关的生物合成基因簇(BGC)。此外,这些菌株携带了从53到152的大量CAZyme,这也通过对多糖纤维素降解的体外分析得到了证明,甲壳素,laminarin,淀粉,还有木聚糖.此外,所有菌株都携带重金属代谢基因,并表现出对重金属的耐受性,在Co2+(3-6)范围内的最小抑制浓度(MIC),以毫摩尔(mM)为单位,Cu2+(0.2-0.4),Ni2+(3-5),Zn2+(2-4),Mn2+(20-50),和Hg2+(0.3)。
结论:基于多相分类方法,这三个分离的菌株代表了两个新物种,名为Marivirgaarenosasp。11月。(BKB1-2T=KCTC82989T=InaCCB1618T),和Marivirgasalinaesp.11月。(BDSF4-3T=KCTC82973T=InaCCB1619T)。
BACKGROUND: The phylum Bacteroidota represents a significant proportion of heterotrophic bacteria found in marine ecosystems. Members of the phylum Bacteroidota are actively involved in the degradation of biopolymers such as polysaccharides and proteins. Bacteroidota genomes exhibit a significant enrichment of various enzymes, including carbohydrate-active enzymes (CAZymes), carboxypeptidases, esterases, isomerases, peptidases, phosphatases, and sulfatases. The genus Marivirga, a member of the family Marivirgaceae within the phylum Bacteroidota, comprises six documented species. During a microbial diversity study, three novel Marivirga strains (BKB1-2 T, ABR2-2, and BDSF4-3 T) were isolated from the West Sea, Republic of Korea.
RESULTS: To explore the taxonomic status and genomic characteristics of the novel isolates, we employed a polyphasic taxonomic approach, which included phylogenetic, chemotaxonomic and comprehensive genome analysis. The three isolates were Gram-stain-negative, aerobic, rod-shaped, moderately halophilic, and had a gliding motility. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the two isolates, BKB1-2 T and BDSF4-3 T, and the six reference strains were 70.5-76.5% for ANI and 18.1-25.7% for dDDH. Interestingly, the Kyoto Encyclopedia of Genes and Genomes (
KEGG) analysis showed that the strains harbor genes for a comprehensive pathway for dissimilatory nitrate reduction to ammonium (DNRA), as well as other nitrogen pathways for the reduction of nitrite, nitric oxide, and nitrous oxide. Additionally, the antiSMASH analysis indicated that the strains contained three to eight biosynthetic gene clusters (BGCs) associated with the synthesis of secondary metabolites. Furthermore, the strains carried a high number of CAZyme ranging from 53 to 152, which was also demonstrated by an in vitro analysis of degradation of the polysaccharide cellulose, chitin, laminarin, starch, and xylan. Additionally, all the strains carried genes for the metabolism of heavy metals, and exhibited tolerance to heavy metals, with minimum inhibitory concentrations (MICs) in millimoles (mM) in ranges of Co2+ (3-6), Cu2+ (0.2-0.4), Ni2+ (3-5), Zn2+ (2-4), Mn2+ (20-50), and Hg2+ (0.3).
CONCLUSIONS: Based on polyphasic taxonomic approach, the three isolated strains represent two novel species names Marivirga arenosa sp. nov. (BKB1-2 T = KCTC 82989 T = InaCC B1618T), and Marivirga salinae sp. nov. (BDSF4-3 T = KCTC 82973 T = InaCC B1619T).