目的:探讨全身照射(TBI)和全腹部照射(WAI)致致命性肠损伤的剂量,健康C57BL/6J小鼠随机分为7组:对照组;6、7和8GyTBI组;5、10和15GyWAI组。生存的长度,一般条件,体重,观察小鼠每日食物和水的摄入量以及小肠的组织病理学变化。
结果:C57BL/6J小鼠的致命性损伤是由≥6GyTBI和15GyWAI引起的。他们的体重和食物摄入量减少,它们的小肠绒毛结构被破坏了,≥6GyTBI组和15GyWAI组空肠周围存活隐窝数量减少。10GyWAI组中的小鼠在5天内体重显著减轻,但此后缓慢恢复。他们的食欲不振,肠粘膜可逆受损。
结论:10GyWAI可引起非致命性肠损伤,而小鼠≥6GyTBI和>15GyWAI可引发致命性肠损伤。本研究结果为建立C57BL/6J小鼠放射性肠损伤模型提供了依据。
OBJECTIVE: To investigate the doses of total body (TBI) and whole abdominal irradiation (WAI) induced lethal intestinal injury, healthy C57BL/6 J mice were divided randomly into 7 groups: control group; 6, 7, and 8 Gy TBI groups; and 5, 10, and 15 Gy WAI groups. The survival length, general conditions, body weight, daily food and water intake of the mice and the histopathological changes of small intestine were observed.
RESULTS: Lethal injury among C57BL/6 J mice was caused by ≥6 Gy TBI and 15 Gy WAI. Their body weight and food intake decreased, the structure of their small intestinal villi was destroyed, and the number of surviving crypts per circumference of the jejunum decreased in ≥6 Gy TBI groups and 15 Gy WAI group. The mice in the 10 Gy WAI group significantly lost weight within 5 days but recovered slowly thereafter. They also had poor appetite and reversibly damaged intestinal mucosa.
CONCLUSIONS: Nonlethal intestinal injury could be induced by 10 Gy WAI, whereas lethal intestinal injury could be triggered by ≥6 Gy TBI and >15 Gy WAI in mice. Our results provided a basis for establishing radiation-induced intestinal injury models with C57BL/6 J mice.