Abstract：
BACKGROUND: Eradication of endodontic biofilms from the infected root canal system is still the main concern in endodontics. In this study, the role of the power density parameter in the efficacy of antimicrobial photodynamic therapy (PDT) with toluidine blue O (TBO) and phycocyanin (PC) activated by a 635 nm diode laser (DL) against Enterococcus faecalis biofilm in the root canal model was investigated.
METHODS: The E. faecalis biofilm in the root canal was treated with TBO and PC with different power densities (636, 954, 1273, and 1592 W/cm2). The untreated biofilm represented the control group. After the treatments, the biofilms were analyzed based on the number of colonies per milliliter.
RESULTS: TBO and PC activated with 635 nm DL with a power density of 1592 W/cm2 were more efficient in removing E. faecalis biofilms within the root canals than those with a power density of 636 W/cm2 (p = 0.00).
CONCLUSIONS: The light power density optimized the bacterial reduction of E. faecalis biofilms in the root canal spaces. These results provide information on the decisive parameters for performing PDT on intracanal biofilms.
METHODS: The E. faecalis biofilm in the root canal was treated with TBO and PC with different power densities (636, 954, 1273, and 1592 W/cm2). The untreated biofilm represented the control group. After the treatments, the biofilms were analyzed based on the number of colonies per milliliter.
RESULTS: TBO and PC activated with 635 nm DL with a power density of 1592 W/cm2 were more efficient in removing E. faecalis biofilms within the root canals than those with a power density of 636 W/cm2 (p = 0.00).
CONCLUSIONS: The light power density optimized the bacterial reduction of E. faecalis biofilms in the root canal spaces. These results provide information on the decisive parameters for performing PDT on intracanal biofilms.
摘要：
背景：从受感染的根管系统中消除牙髓生物膜仍然是牙髓学的主要关注点。在这项研究中,研究了能量密度参数在通过635nm二极管激光（DL）激活的甲苯胺蓝O（TBO）和藻蓝蛋白（PC）对抗根管模型中粪肠球菌生物膜的抗菌光动力疗法（PDT）疗效中的作用。
方法：用不同剂量的光能（600、900、1200和1500J/cm2）的TBO和PC处理根管中的粪肠球菌生物膜。未处理的生物膜代表对照组。治疗后,基于每毫升菌落数分析生物膜。
结果：用能量密度为1500J/cm2的635nmDL激活的TBO和PC比能量密度为600J/cm2的TBO和PC更有效地去除根管内的粪肠球菌生物膜（p=0.00）。
结论：光能量剂量优化了根管空间中粪肠球菌生物膜的细菌减少。这些结果提供了对肛门内生物膜进行PDT的决定性参数的信息。
方法：用不同剂量的光能（600、900、1200和1500J/cm2）的TBO和PC处理根管中的粪肠球菌生物膜。未处理的生物膜代表对照组。治疗后,基于每毫升菌落数分析生物膜。
结果：用能量密度为1500J/cm2的635nmDL激活的TBO和PC比能量密度为600J/cm2的TBO和PC更有效地去除根管内的粪肠球菌生物膜（p=0.00）。
结论：光能量剂量优化了根管空间中粪肠球菌生物膜的细菌减少。这些结果提供了对肛门内生物膜进行PDT的决定性参数的信息。
