本研究旨在系统地研究野生和养殖黄翅目之间肉品质的潜在差异。平均体重45g,每组15例健康M.albus样本,通过生化指标分析肌肉参数。组织形态学,和分子生物学。与野生鱼相比,肉中养殖的M.albus具有较低的粗蛋白,胶原蛋白,赖氨酸,组氨酸,总氨基酸,SFA,n-3PUFA含量,和n-3/n-6比值(p<0.05),和更高的水分,粗脂,粗灰分,MUFA,n-6PUFA,和总PUFA含量(p<0.05)。解冻损失,滴水损失,蒸汽损失,养殖组的沸腾损失明显更高,和硬度,弹性,凝聚力,发胶,耐嚼,恢复力和恢复力均显著低于野生组(p<0.05)。此外,在野生M.albus中观察到较高的肌纤维密度和较低的肌纤维直径(p<0.05)。在肌肉转录组分析中,差异表达基因和富集途径主要与肌肉发育有关,蛋白质合成,分解代谢,脂质代谢,和豁免权。据我们所知,这是第一次在生物化学方面比较野生和养殖的M.albus之间的肉质量的调查,组织学,和分子生物学水平。总的来说,野生M.albus比养殖M.albus具有更高的营养价值和质地品质。
The present study aimed to systematically investigate the underlying differences in flesh quality between wild and farmed Monopterus albus. Fifteen healthy M. albus per group with an average body weight of 45 g were sampled to analyze muscle parameters by biochemical indicators, histomorphology, and molecular biology. Compared with the wild fish, the farmed M. albus in flesh had lower crude protein, collagen, lysine, histidine, total amino acids, SFA, n-3 PUFA contents, and n-3/n-6 ratio (p < 0.05), and higher moisture, crude lipid, crude ash, MUFA, n-6PUFA, and total PUFA contents (p < 0.05). The thawing loss, drip loss, steaming loss, and boiling loss in the farmed group were significantly higher, and hardness, springiness, cohesiveness, gumminess, chewiness, and resilience were significantly lower than those in the wild group (p < 0.05). In addition, higher muscle fiber density and lower muscle fiber diameter were observed in wild M. albus (p < 0.05). In muscle transcriptome profiling, differentially expressed genes and enriched pathways are primarily associated with muscle development, protein synthesis, catabolism, lipid metabolism, and immunity. To the best of our knowledge, this is the first investigation that compares the flesh quality between wild and farmed M. albus in terms of biochemistry, histology, and molecular biology levels. Overall, wild M. albus had a higher nutritional value and texture quality than farmed M. albus.