UNASSIGNED:建立IncFIB复制子的分型方案并解剖IncFIB-4.1/4.2单复制子质粒的基因组特征。
UNASSIGNED:选择总共146个代表性的完全测序的含IncFIB-复制子的质粒来构建repBIncFIB序列的系统发育树。这里对来自中国的9个IncFIB-4.1/4.2单复制子质粒进行了完整测序,并与来自GenBank的第一个测序的IncFIB-4.1/4.2单复制子质粒进行了比较,以剖析其基因组多样性。
未经批准:在这项研究中,提出了一种基于repB序列的方案,用于将IncFIB复制子分为7种主要类型,并进一步分为70种亚型。此处对9个IncFIB-4.1/4.2单复制子质粒的集合进行完全测序,并与来自GenBank的第一个测序的IncFIB-4.1/4.2单复制子质粒进行比较。这11个质粒具有小的骨架,并且仅共享三个关键的骨架标记repB及其迭代,parABC,和stbd。每个质粒都包含一个插入到主链中的大辅助区(LAR),这11个LARs具有明显不同的可移动遗传元件(MGEs)和抗性/代谢基因位点。在这11个LAR中的7个中发现了抗生素抗性区域(ARR;含抗生素抗性基因的遗传元件)。除了抗性基因,ARR携带单位或复合转座子,积分子,和假定的阻力单位。IncFIB-4.1/4.2单复制子质粒是耐药基因的重要载体。这是三个小说MGE的第一份报告:In1776,Tn6755和Tn6857。
UNASSIGNED:此处提供的数据为IncFIB复制子和IncFIB-4.1/4.2单复制子质粒的多样性和进化提供了更深入的见解。
UNASSIGNED: To establish a typing scheme for
IncFIB replicon and to dissect genomic features of
IncFIB-4.1/4.2 single-replicon plasmids.
UNASSIGNED: A total of 146 representative fully sequenced IncFIB-replicon-containing plasmids were selected to construct a phylogenetic tree of repB
IncFIB sequences. A collection of nine IncFIB-4.1/4.2 single-replicon plasmids from China were fully sequenced here and compared with the first sequenced IncFIB-4.1/4.2 single-replicon plasmids from GenBank to dissect their genomic diversity.
UNASSIGNED: In this study, a repB sequence-based scheme was proposed for grouping IncFIB replicon into seven primary types and further into 70 subtypes. A collection of nine IncFIB-4.1/4.2 single-replicon plasmids were fully sequenced here and compared with the first sequenced
IncFIB-4.1/4.2 single-replicon plasmids from GenBank. These 11 plasmids had small backbones and shared only three key backbone markers repB together with its iterons, parABC, and stbD. Each plasmid contained one large accessory region (LAR) inserted into the backbone, and these 11 LARs had significantly distinct profiles of mobile genetic elements (MGEs) and resistance/metabolism gene loci. Antibiotic resistance regions (ARRs; the antibiotic resistance gene-containing genetic elements) were found in seven of these 11 LARs. Besides resistance genes, ARRs carried unit or composite transposons, integrons, and putative resistance units. IncFIB-4.1/4.2 single-replicon plasmids were important vectors of drug resistance genes. This was the first report of three novel MGEs: In1776, Tn6755, and Tn6857.
UNASSIGNED: Data presented here provided a deeper insight into diversity and evolution of IncFIB replicon and
IncFIB-4.1/4.2 single-replicon plasmids.