PDF(Pubmed)
Abstract：
Carbapenem-resistant Salmonella enterica (S. enterica) pose a significant threat to public health, causing gastroenteritis and invasive infections. We report the first emergence of a carbapenem-resistant S. enterica serovar London strain, A132, carrying the blaNDM-5 gene in China. Whole-genome sequencing and bioinformatics analysis assigned A132 to be ST155, a multidrug-resistant clone frequently reported in China. The strain A132 exhibited resistance to multiple antibiotics, with 20 acquired antibiotic resistance genes (ARGs) identified, predominantly located on the IncFIB plasmid (pA132-1-NDM). Notably, the blaNDM-5 gene was located within an IS26 flanked-class 1 integron-ISCR1 complex, comprising two genetic cassettes. One cassette is the class 1 integron, which may facilitate the transmission of the entire complex, while the other is the blaNDM-5-containing ISCR1-IS26-flanked cassette, carrying multiple other ARGs. Genbank database search based on the blaNDM-5-carrying cassette identified a similar genetic context found in transmissible IncFIA plasmids from Escherichia coli (p91) and Enterobacter hormaechei (p388) with a shared host range, suggesting the potential for cross-species transmission of blaNDM-5. To our knowledge, this is the first reported case of Salmonella serovar London ST155 harboring blaNDM-5 gene. Phylogenetic analysis indicated a close relationship between A132 and eight S. London ST155 strains isolated from the same province. However, A132 differed by carrying the blaNDM-5 gene and four unique ARGs. Given the high transmissibility of the F-type plasmid harboring blaNDM-5 and 18 other ARGs, it is imperative to implement vigilant surveillance and adopt appropriate infection control measures to mitigate the threat to public health.
摘要：
耐碳青霉烯类沙门氏菌(S.enterica)对公共卫生构成重大威胁，引起胃肠炎和侵袭性感染.我们报道了耐碳青霉烯的肠链球菌血清London菌株的首次出现，A132，在中国携带blaNDM-5基因。全基因组测序和生物信息学分析将A132指定为ST155，这是中国经常报道的多药耐药克隆。菌株A132表现出对多种抗生素的抗性,确定了20个获得性抗生素抗性基因（ARGs），主要位于IncFIB质粒(pA132-1-NDM)上。值得注意的是,blaNDM-5基因位于IS26侧翼的1类整合子-ISR1复合物中，包含两个基因盒。一个卡带是1类整合子，这可能有助于整个建筑群的传输，而另一个是含有blaNDM-5的ISR1-IS26侧翼盒，携带多个其他ARG。基于携带blaNDM-5的盒的Genbank数据库搜索确定了在大肠杆菌（p91）和ormaechei肠杆菌（p388）的可传播的IncFIA质粒中发现的相似遗传背景，具有共享的宿主范围，提示blaNDM-5跨物种传播的可能性。据我们所知,这是首例报道的携带blaNDM-5基因的沙门氏菌伦敦ST155。系统发育分析表明，从同一省分离的A132和8个伦敦ST155菌株之间存在密切的关系。然而,A132的不同之处在于携带blaNDM-5基因和四个独特的ARG。鉴于携带blaNDM-5和18种其他ARG的F型质粒的高传播性，必须实施警惕的监测和采取适当的感染控制措施，以减轻对公众健康的威胁。
