本研究评估了发酵Limosilactacillus对环磷酰胺(CP)诱导的BALB/c小鼠免疫抑制的免疫增强作用。体外,采用ELISA和Westernblot分析Raw264.7细胞中促炎细胞因子和MAPK信号分子的表达。此外,细胞增殖,表面受体表达,和NK-92细胞的细胞毒性通过细胞计数试剂盒-8,CytoTox96测定,和流式细胞术,分别。研究发酵乳杆菌菌株的体内免疫增强作用。这些菌株口服给BALB/c小鼠2周,腹膜内注射CP。然后,肝脏,脾,脾从每只动物中分离全血。施用单一发酵乳杆菌菌株或其混合物可维持脾脏重量,白细胞计数与非饲喂组相比。所有发酵乳杆菌饲喂组的脾细胞增殖和NK细胞毒性均显着增加。在从发酵乳杆菌组分离的脾细胞中,B220+细胞的频率也显著提高。此外,细胞因子的产生(TNF-α,IFN-γ)和抗体在从发酵乳杆菌组分离的脾细胞上清液中回收。总之,发酵乳杆菌可能是一种合适的具有免疫增强作用的功能性食品添加剂。
This study evaluates the immune-enhancing effects of Limosilactobacillus fermentum on cyclophosphamide (CP)-induced immunosuppression in BALB/c mice. In vitro, the expressions of pro-inflammatory cytokines and MAPK signaling molecules in Raw264.7 cells were analyzed by ELISA and Western blot analysis. Moreover, cell proliferation, surface receptor expression, and cytotoxicity of NK-92 cells were examined by Cell Counting Kit-8, CytoTox96 assay, and flow cytometry, respectively. To investigate the immune-enhancing effects of selected L. fermentum strains in vivo, these strains were orally administered to BALB/c mice for 2 weeks, and CP was intraperitoneally injected. Then, liver, spleen, and whole blood were isolated from each animal. Administration of single L. fermentum strains or their mixture sustained the spleen weight, the counts of white blood cells compared to non-fed group. Splenocyte proliferation and NK cytotoxicity were significantly increased in all L. fermentum-fed groups. The frequency of B220+ cells was also significantly enhanced in splenocytes isolated from L. fermentum groups. In addition, the production of cytokines (TNF-α, IFN-γ) and antibodies was recovered in splenocyte supernatants isolated from L. fermentum groups. In conclusion, L. fermentum could be a suitable functional food additive for immune-enhancing effect.