Abstract：
To alleviate the adverse effects of chemotherapy and bolster immune function, a novel polysaccharide derived from Sargassum fusiforme named as SFP-αII. The structural composition of SFP-αII predominantly consisted of guluronic and mannuronic acids in a molar ratio of 33.8:66.2, with an average molecular weight of 16.5 kDa. Its structure was primarily characterized by →4)-α-GulA-(1 → and →4)-β-ManA-(1 → linkages confirmed by FT-IR, methylation, and NMR analyses. The absence of a triple-helix structure was in SFP-αII was confirmed using circular dichroism and Congo red dye assays. The dimensions varied with lengths ranging from 20 nm up to 3 μm revealed by atomic force microscopy (AFM). SFP-αII has been found to enhance immunomodulatory activity in cyclophosphamide (CTX)-induced immunosuppressed mice. This was evidenced by improvements in immune organ indices, cytokine levels, and the release of nitric oxide (NO). Specifically, SFP-αII mitigated immunosuppression by upregulating the secretion of IL-1β (167.3 %) and TNF-α (227.1 %) at a dose of 400 mg/kg, compared with the CTX group in macrophages. Ultimately, SFP-αII may serve as a mechanism for immune enhancement through modulation of TLR4-mediated NF-κB and MAPK signaling pathways. This integration of traditional Chinese and Western medicine, leveraging SFP-αII as a potential functional food could be pivotal in alleviating immunosuppressive side effects in CTX treatment.
摘要：
为了减轻化疗的不良反应，增强免疫功能，一种来源于羊尾藻的新型多糖，命名为SFP-αII。SFP-αII的结构组成主要由古洛糖醛酸和甘露糖醛酸组成，摩尔比为33.8：66.2，平均分子量为16.5kDa。其结构主要表征为→4）-α-GulA-（1→和→4）-β-ManA-（1→通过FT-IR确认的连接，甲基化,和NMR分析。使用圆二色性和刚果红染料测定证实了SFP-αII中不存在三螺旋结构。尺寸随原子力显微镜（AFM）显示的20nm至3μm的长度而变化。已经发现SFP-αII在环磷酰胺(CTX)诱导的免疫抑制小鼠中增强免疫调节活性。免疫器官指数的改善证明了这一点，细胞因子水平，和一氧化氮（NO）的释放。具体来说,SFP-αII通过上调400mg/kg剂量的IL-1β（167.3％）和TNF-α（227.1％）的分泌来减轻免疫抑制，与CTX组巨噬细胞比拟。最终,SFP-αII可能通过调节TLR4介导的NF-κB和MAPK信号通路作为免疫增强机制。这种中西医结合,在CTX治疗中,利用SFP-αII作为一种潜在的功能性食物可能是减轻免疫抑制副作用的关键.
