■探讨Tregs通过ICOS诱导肿瘤相关巨噬细胞极化为M2型促进结直肠癌进展的机制。
■收集268例初次手术的结直肠癌患者术后病理组织及临床病理资料。免疫组织化学(IHC)检测ICOS的表达水平,CD163(M2巨噬细胞的标志物),和Foxp3(Tregs的标记)在癌症中,相邻的非肿瘤,和正常组织。ICOS的关系,M2巨噬细胞,和Tregs在CRC中的临床病理特征和术前肿瘤标志物(如CEA和CA199)进行了探讨。
■M2巨噬细胞和Tregs的表达水平随肿瘤进展而增加,而ICOS表达呈下降趋势。与邻近组织和正常组织相比,ICOS的表达水平,M2巨噬细胞,CRC组织中Tregs较高。巨噬细胞M2和Tregs的表达水平与肿瘤标志物呈显著正相关,而ICOS表达呈显著负相关。
■Tregs和ICOS诱导的肿瘤相关m2巨噬细胞参与结直肠癌肿瘤微环境的动态平衡,它们的相互作用影响结直肠癌的发生和进展。高水平的ICOS与更好的长期生存率相关。
UNASSIGNED: To explore the mechanism by which Tregs promote the progression of colorectal cancer by inducing tumor-associated macrophages to polarize into M2 type via
ICOS.
UNASSIGNED: Postoperative pathological tissues and clinical pathological data of 268 colorectal cancer patients who underwent initial surgery were collected. Immunohistochemistry (IHC) was used to detect the expression levels of ICOS, CD163 (a marker for M2 macrophages), and Foxp3 (a marker for Tregs) in cancerous, adjacent non-tumorous, and normal tissues. The relationship of
ICOS, M2 macrophages, and Tregs in CRC with clinical pathological characteristics and pre-surgical tumor markers (such as CEA and CA199) was explored.
UNASSIGNED: The expression levels of M2 macrophages and Tregs increased with tumor progression, while ICOS expression showed a decreasing trend. Compared to adjacent and normal tissues, the expression levels of
ICOS, M2 macrophages, and Tregs were higher in CRC tissues. The expression levels of M2 macrophages and Tregs were significantly positively correlated with tumor markers, while
ICOS expression was significantly negatively correlated.
UNASSIGNED: Tumor-associated m2 macrophages induced by Tregs and ICOS participate in the dynamic balance of the colorectal cancer tumor microenvironment, and their interaction affects colorectal carcinogenesis and progression. High levels of
ICOS are associated with better long-term survival rates.