■小B细胞淋巴瘤(SBCL)的临床和病理鉴别诊断仍然存在争议,并且可能会在其重叠形态上造成困难。表型,分化为浆细胞。我们的目的是检查标志物βmune受体易位相关蛋白1(IRTA1)的表达,髓系细胞核分化抗原(MNDA),在涉及可能具有浆细胞分化的不同部位的SBCL病例中,淋巴增强子结合因子1(LEF1)和stathmin1。
■我们研究了154个涉及的组织样本,来自116名患者,并评估了标记物的染色分布。检测21例慢性淋巴细胞白血病/淋巴瘤(CLL/SLL)的表达,7滤泡性淋巴瘤(FL),14结节边缘区淋巴瘤(NMZL),17结外边缘区淋巴瘤(ENMZL),55脾边缘区淋巴瘤(SMZL),22例边缘区淋巴瘤-未指明(MZL-NOS)和18例淋巴浆细胞性淋巴瘤/Waldenström巨球蛋白血症(LPL/WS)。
■结果证实,LEF1是CLL/SLL和Stathmin对FL的最敏感和特异性标记(p<0.001)。MNDA和IRTA1是区分边缘区淋巴瘤的有用标记。
■我们的结果表明,CLL/SLL的LEF1和FL的Stathmin是可靠的标记。LEF1,MNDA,考虑到它们的局限性,Stathmin1和IRTA1有助于诊断算法中其他常规使用的免疫组织化学标记。
UNASSIGNED: Clinical and pathological differential diagnosis of small B-cell lymphomas (SBCLs) is still controversial and may create difficulty with their overlapping morphology, phenotype, differentiation to plasma cells. We aimed to examine the expression of the markers ımmune receptor translocation -associated protein 1 (
IRTA1), myeloid cell nuclear differentiation antigen (MNDA), lymphoid enhancer-binding factor-1 (LEF1) and stathmin1 in SBCL cases involving different sites which may have plasma cell differentiation.
UNASSIGNED: We studied 154 involved tissue samples, from 116 patients and evaluated the staining distribution of the markers. The expressions were evaluated on 21 chronic lymphocytic leukemia/ lymphoma (CLL/SLL), 7 follicular lymphoma (FL), 14 nodal marginal zone lymphoma (NMZL), 17 extranodal marginal zone lymphoma (ENMZL), 55 splenic marginal zone lymphoma (SMZL), 22 marginal zone lymphoma -not otherwise specified (MZL-NOS) and 18 lymphoplasmacytic lymphoma/Waldenström\'s macroglobulinemia (LPL/WS) cases by immunohistochemistry.
UNASSIGNED: The results confirmed that LEF1 was the most sensitive and specific markers for CLL/SLL and Stathmin for FL (p <0.001). MNDA and
IRTA1 were useful markers to distinguish marginal zone lymphomas.
UNASSIGNED: Our results suggest LEF 1 for CLL/SLL and Stathmin for FL are reliable markers. LEF1, MNDA, Stathmin1 and
IRTA1 are helpful with other routinely used immunohistochemical markers in a diagnostic algorithm considering their limitations.