单核细胞增生李斯特菌(L.单核细胞增多症)是一种病原体,通过受污染的食物传播,并导致称为李斯特菌病的疾病。毒力因子InlA在单核细胞增生李斯特菌侵入人肠上皮细胞中起着至关重要的作用。此外,InlA增强宿主菌株的致病性,和不同的单核细胞增生李斯特菌菌株含有不同的InlA变异。我们的研究分析了来自511种不同来源的序列类型(STs)的总共4393个已发表的单核细胞增生李斯特菌基因组。我们鉴定了300种独特的InlA蛋白序列类型(PST),并揭示了45种高度突变的氨基酸位点。发现富含亮氨酸的重复序列(LRR)区域在InlA中最保守,而蛋白A(PA)区的突变率最高。在InlA的B-重复区中鉴定出两种新类型的突变。对应分析(CA)用于分析谱系或10种最常见的序列类型(ST)与氨基酸(aa)位点之间的相关性。ST8与位点192_F密切相关,454_T.ST7与位点51_A有很强的相关性,573_E,648_S,和664_A,它也与ST6和站点544_N相关,671_A,738_B,739_B,740_B,和774_Y.此外,ST1和站点142_S之间有很强的相关性,738_N,ST2和站点2_K,142_S,738_N,以及ST87和site2_K,738_N被证明。我们的发现极大地有助于理解分布,composition,InlA在单核细胞增生李斯特菌中的保守性。这些发现还表明了InlA在支持分子流行病学追踪工作中的潜在作用。
Listeria monocytogenes (L. monocytogenes) is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor
InlA plays a crucial role in the invasion of L. monocytogenes into the human intestinal epithelium. In addition,
InlA enhances the pathogenicity of host strains, and different strains of L. monocytogenes contain varying variations of
InlA. Our study analyzed a total of 4393 published L. monocytogenes genomes from 511 sequence types (STs) of diverse origins. We identified 300 unique
InlA protein sequence types (PSTs) and revealed 45 highly mutated amino acid sites. The leucine-rich repeat (LRR) region was found to be the most conserved among the InlA, while the protein A (PA) region experienced the highest mutation rate. Two new types of mutations were identified in the B-repeat region of
InlA. Correspondence analysis (CA) was used to analyze correlations between the lineages or 10 most common sequence types (STs) and amino acid (aa) sites. ST8 was strongly correlated with site 192_F, 454_T. ST7 exhibited a strong correlation with site 51_A, 573_E, 648_S, and 664_A, and it was also associated with ST6 and site 544_N, 671_A, 738_B, 739_B, 740_B, and 774_Y. Additionally, a strong correlation between ST1 and site 142_S, 738_N, ST2 and site 2_K, 142_S, 738_N, as well as ST87 and site2_K, 738_N was demonstrated. Our findings contribute significantly to the understanding of the distribution, composition, and conservation of InlA in L. monocytogenes. These findings also suggest a potential role of InlA in supporting molecular epidemiological tracing efforts.