背景:NK细胞在免疫反应中起重要作用,免疫监视,和新陈代谢调节。因此,NK细胞参与各种疾病的发生发展,如传染病,癌症,肥胖,和糖尿病。IL-25是具有抗炎功能的IL-17家族的特殊成员。IL-25可以通过多种免疫细胞调节炎症反应和代谢;IL-25在NK细胞中的作用及调控机制尚不清楚。
方法:在本研究中,我们通过4D无标记质谱研究IL-25在NK细胞蛋白谱中的作用,并通过PRM分析验证差异蛋白.此外,GO分析,KEGG分析,和其他生物信息学分析方法用于探索差异表达蛋白的富集功能和信号通路。
结论:GO和KEGG分析表明IL-25可能会影响这些过程,比如新陈代谢,产热,和NK细胞的氧化磷酸化。有7种下调蛋白(NCR1,GZMB,与对照组相比,IL-25处理的NK细胞中的PRF1,KLRC1,NDUFA11,LAMTOR5和IKBIP)和1个上调蛋白(PSMD7)用于PRM验证。我们的结果表明,IL-25可能通过NK细胞调节代谢和其他生物学过程,这将有利于揭示IL-25在各种疾病中NK细胞中的作用和调控机制。
结论:蛋白质组学结合生物信息学分析将有助于挖掘隐藏在质谱数据背后的更多信息,为寻找临床生物标志物和疾病机制奠定基础。
BACKGROUND: NK cells play an important role in immune response, immune surveillance, and metabolism regulation. Therefore, NK cells are involved in the occurrence and development of various diseases, such as infectious diseases, cancer, obesity, and diabetes. IL-25 is a special member of the IL-17 family with anti-inflammatory function. IL-25 can regulate inflammatory response and metabolism via various immune cells; however, the role and regulatory mechanism of IL-25 in NK cells are still unclear.
METHODS: In this study, we investigate the role of IL-25 in NK-cell protein profile via 4D label-free mass spectrum and validate the differential proteins via PRM analysis. In addition, GO analysis, KEGG analysis, and other bioinformatic analysis methods are used to explore the enriched function and signal pathway of differentially expressed proteins.
CONCLUSIONS: The GO and KEGG analyses suggest that IL-25 may affect the processes, such as metabolism, thermogenesis, and oxidative phosphorylation of NK cells. There are 7 down-regulated proteins (NCR1, GZMB, PRF1, KLRC1, NDUFA11, LAMTOR5, and IKBIP) and 1 up-regulated protein (PSMD7) in IL-25-treated NK cells versus the control group for PRM validation. Our results indicate that IL-25 may regulate metabolism and other biological processes via NK cells, which will be beneficial in revealing the role and regulatory mechanisms of IL-25 in NK cells in various diseases.
CONCLUSIONS: Proteomics combined with bioinformatic analysis will help to mine more information hidden behind mass spectrometry data and lay the foundation for finding clinical biomarkers and mechanisms of diseases.