■囊性棘球蚴病(CE)是由and虫引起的寄生虫人畜共患病。在过去的几年里,大量的研究已经做了关于肝脏疾病使用代谢组学技术来识别生物标志物,可以识别疾病的早期阶段。本研究旨在探索血清中的生物标志物,尿液,和囊性液体有助于分化,分期,并使用质子核磁共振(1HNMR)代谢组学评估腹内包虫囊肿的生育力。
■在研究中,纳入28名受试者(16例和12例对照)。使用超声检查对包虫囊肿进行分期。在符合病例和对照定义的患者中,血,尿液,收集囊液进行全血细胞计数,尿培养,棘球蚴IgG酶联免疫吸附试验(ELISA),和代谢组学分析。血清中的17、15和11代谢物,尿液,并对囊性液体样本进行定量,分别,区分病例组和对照组。
■在这项研究中,我们观察到在病例的尿液样本中琥珀酸代谢物有显著的下调,下调五种代谢物(异亮氨酸,缬氨酸,组氨酸,酪氨酸和甲酸)和病例囊性液中丙氨酸的上调。
■目前的研究表明,代谢组学可以非侵入性地用于CE的快速诊断。这是极少数的研究之一,使用1HNMR光谱,分析血清中代谢物的分布,尿液,CE和对照病例中的囊性液体。
■RajN,潘迪A,罗伊·R,etal.血清质子核磁共振(1HNMR)代谢组学研究,尿液,和囊性液体用于区分成人腹内包虫囊肿的生育力和分期。欧亚J肝胃肠病2024;14(1):30-34。
UNASSIGNED: Cystic echinococcosis (CE) is a parasitic zoonosis caused by the tapeworm Echinococcus granulosus. Over the past few years, a lot of research has been done on liver illnesses using metabolomics techniques to identify biomarkers which could identify the diseases in its early stages. The present study was done to explore biomarkers in serum, urine, and cystic fluid which would help in differentiating, staging, and assessing fertility of intra-abdominal hydatid cyst by using proton nuclear magnetic resonance (1H NMR) metabolomics.
UNASSIGNED: In the study, 28 subjects (16 cases and 12 controls) were enrolled. Staging of hydatid cysts was performed using ultrasonography. In patients complying with case and control definition, blood, urine, and cystic fluid were collected for complete blood count, urine culture, Echinococcus IgG enzyme-linked immunosorbent assay (ELISA), and metabolomic analysis. The 17, 15, and 11 metabolites in serum, urine, and cystic fluid samples were quantified, respectively, to differentiate between case and control group.
UNASSIGNED: In this study, we observed that there was a significant downregulation of succinate metabolite in urine samples of cases, down-regulation of five metabolites (isoleucine, valine, histidine, tyrosine and formate) and upregulation of alanine in cystic fluid of cases.
UNASSIGNED: Current study demonstrates that metabolomics can be used non-invasively for rapid diagnosis of CE. This is one of the very few studies, which used 1H NMR spectroscopy, to analyze the profile of metabolites in serum, urine, and cystic fluid in cases of CE and controls.
UNASSIGNED: Raj N, Pandey A, Roy R, et al. Proton Nuclear Magnetic Resonance (1H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults. Euroasian J Hepato-Gastroenterol 2024;14(1):30-34.