PDF(Pubmed)
Abstract：
There is a significant focus on the role of the host microbiome in different outcomes of human parasitic diseases, including cystic echinococcosis (CE). This study was conducted to identify the intestinal microbiome of patients with CE at different stages of hydatid cyst compared to healthy individuals. Stool samples from CE patients as well as healthy individuals were collected. The samples were divided into three groups representing various stages of hepatic hydatid cyst: active (CE1 and CE2), transitional (CE3), and inactive (CE4 and CE5). One family member from each group was selected to serve as a control. The gut microbiome of patients with different stages of hydatid cysts was investigated using metagenomic next-generation amplicon sequencing of the V3-V4 region of the 16S rRNA gene. In this study, we identified 4862 Operational Taxonomic Units from three stages of hydatid cysts in CE patients and healthy individuals with a combined frequency of 2,955,291. The most abundant genera observed in all the subjects were Blautia, Agathobacter, Faecalibacterium, Bacteroides, Bifidobacterium, and Prevotella. The highest microbial frequency was related to inactive forms of CE, and the lowest frequency was observed in the group with active forms. However, the lowest OTU diversity was found in patients with inactive cysts compared with those with active and transitional cyst stages. The genus Agatobacter had the highest OTU frequency. Pseudomonas, Gemella, and Ligilactobacillus showed significant differences among the patients with different stages of hydatid cysts. Additionally, Anaerostipes and Candidatus showed significantly different reads in CE patients compared to healthy individuals. Our findings indicate that several bacterial genera can play a role in the fate of hydatid cysts in patients at different stages of the disease.
摘要：
在人类寄生虫病的不同结局中，宿主微生物组有重要的作用。包括囊性包虫病（CE）。进行这项研究是为了确定与健康个体相比，处于包虫囊肿不同阶段的CE患者的肠道微生物组。收集来自CE患者以及健康个体的粪便样品。样本分为三组，代表肝包虫囊肿的不同阶段：活性（CE1和CE2），过渡（CE3），和非活性（CE4和CE5）。从每个组中选择一个家庭成员作为对照。使用16SrRNA基因V3-V4区的宏基因组下一代扩增子测序研究了包虫囊肿不同阶段患者的肠道微生物组。在这项研究中,我们从CE患者和健康个体的包虫囊肿三个阶段中确定了4862个操作分类单位，合并频率为2,955,291。在所有受试者中观察到的最丰富的属是布劳特氏菌，不动杆菌属,粪杆菌,拟杆菌,双歧杆菌,还有Prevotella.最高的微生物频率与CE的非活性形式有关，在具有活性形式的组中观察到最低的频率。然而,与活动期和移行期囊肿患者相比,非活动期囊肿患者的OTU多样性最低.杆菌属具有最高的OTU频率。假单胞菌,Gemella,在包虫囊肿不同分期的患者中，与利氏杆菌有显著差异。此外,与健康个体相比，厌氧菌和念珠菌在CE患者中显示出明显不同的读数。我们的发现表明，几种细菌属可以在疾病不同阶段的患者中包虫囊肿的命运中发挥作用。
