Microorganisms are a crucial component of lake ecosystems and significant contributors to biogeochemical cycles. However, the understanding of how primary microorganism groups (e.g., bacteria and fungi) are distributed and constructed within different lake habitats is lacking. We investigated the bacterial and fungal communities of Hulun Lake using high-throughput sequencing techniques targeting 16S rRNA and Internal Transcribed Spacer 2 genes, including a range of ecological and statistical methodologies. Our findings reveal that environmental factors have high spatial and temporal variability. The composition and community structures vary significantly depending on differences in habitats. Variance partitioning analysis showed that environmental and geographical factors accounted for <20% of the community variation. Canonical correlation analysis showed that among the environmental factors, temperature, pH, and dissolved oxygen had strong control over microbial communities. However, the microbial communities (bacterial and fungal) were primarily controlled by the dispersal limitations of stochastic processes. This study offers fresh perspectives regarding the maintenance mechanism of bacterial and fungal biodiversity in lake ecosystems, especially regarding the responses of microbial communities under identical environmental stress.IMPORTANCELake ecosystems are an important part of the freshwater ecosystem. Lake microorganisms play an important role in material circulation and energy flow owing to their unique enzymatic and metabolic capacity. In this study, we observed that bacterial and fungal communities varied widely in the water and sediments of Hulun Lake. The primary factor affecting their formation was identified as dispersal limitation during stochastic processes. Environmental and geographical factors accounted for <20% of the variation in bacterial and fungal communities, with pH, temperature, and dissolved oxygen being important environmental factors. Our findings provide new insights into the responses of bacteria and fungi to the environment, shed light on the ecological processes of community building, and deepen our understanding of lake ecosystems. The results of this study provide a reference for lake management and conservation, particularly with respect to monitoring and understanding microbial communities in response to environmental changes.

Hulun Lake is facing significant water quality degradation, necessitating effective monitoring for safety. Traditional methods lack the necessary spatial and temporal coverage, underscoring the need for a remote sensing model. In this study, we utilized the Landsat 8 OLI dataset, incorporating cross-section monitoring and field sampling data comprehensively. Employing the random forest algorithm, we constructed a remote sensing inversion model for six water quality parameters in Hulun Lake: chlorophyll-a (Chl-a), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH3-N), chemical oxygen demand (COD), and dissolved oxygen (DO). The model was applied to the non-freezing period of Hulun Lake from 2016 to 2021, exhibiting commendable performance and generating high-resolution maps. Time series analysis revealed that during the study period, the pollution levels of TN, TP, and COD in Hulun Lake were extremely serious, exceeding the Class V water standard of China\'s surface water environmental quality standard. Regional analysis indicated lower pollutant concentrations in the central lake area compared to the lake inlet. The inflowing rivers with high pollution adversely impacted Hulun Lake\'s water quality. To ensure the continued health of Hulun Lake\'s water quality, it is imperative to monitor lake water quality attentively and implement necessary measures to prevent further deterioration. This study holds crucial importance for shaping and executing ecological protection and restoration strategies for Hulun Lake.

Aquatic fungi form both morphologically and ecologically diverse communities. However, lake ecosystems are frequently overlooked as fungal habitats, despite the potentially important role of fungi in matter cycling and energy flow. Hulun Lake is a typical example of a seasonal glacial lake; however, previous studies have only focused on bacteria in this ecosystem. Therefore, in the current study, internal transcribed spacer ribosomal RNA (ITS rRNA) gene high-throughput sequencing was used to investigate the fungal communities in paired water and sediment samples from the Hulun Lake Basin in China. A significant difference was found between the fungal communities of the two sample types. Across all samples, we identified nine phyla, 30 classes, 78 orders, 177 families, and 307 genera. The dominant phyla in the lake were Ascomycota, Basidiomycota and Chytridiomycota. Our results show that both water and sediments have very high connectivity, are dominated by positive interactions, and have similar interaction patterns. The fungal community structures were found to be significantly affected by environmental factors (temperature, chemical oxygen demand, electrical conductivity, total phosphorus, and pH). In addition, the dispersal limitations of the fungi affected the structure of the fungal communities, and it was revealed that stochasticity is more important than deterministic mechanisms in influencing the structure and function of fungal communities. This study provides unique theoretical support for the study of seasonally frozen lake fungal communities and a scientific basis for the future management and protection of Hulun Lake.

The occurrence, migration, transformation, and stability of sediment (SOM) and suspended particulate (SPOM) organic matters have important effects on the environmental behaviors of carbon, nitrogen, phosphorus, and other pollutants in a water environment. The content, composition, fluorescence characteristics, source, and stability of SOM and SPOM in Hulun Lake, a typical lake in cold and arid region of China, were compared by sequential extraction, three-dimensional fluorescence spectroscopy, parallel factor technique, carbon-nitrogen ratio, and stable carbon isotope. Contents of SOM and SPOM in north and west were higher than those in east and south. The average content of SPOM (24.70 ± 4.63 g/kg) was slightly higher than that of SOM (23.04 ± 10.27 g/kg), but the difference was not significant. Humin was the dominant component in SOM and SPOM, accounting for 73.7% and 61.2%, respectively. Humus was the main fluorescence component of water-extractable organic matter in SOM and SPOM, accounting for 79.9% and 70.4%, respectively, of the total fluorescence intensity. SOM and SPOM were derived from terrestrial sources with a relative contribution rate of about 70%. SPOM was more influenced by autochthonous sources and had a significantly lower humification degree and stability than SOM. Effects of climate changes on migration, transformation, stability, and bioavailability of organic matters and endogenous pollutants closely related to organic matters in lakes of cold and arid regions should be paid attention in the future.

Lake organic matter is one of the important forms of terrestrial carbon, and its sedimentary evolution is affected by many factors such as climate and sources. However, few studies have been conducted on the feedback mechanism of the sedimentary evolution of organic matter to climate change in cold and arid lakes. Historical variations and compositions of sources of the sediment organic matter (SOM) of Hulun Lake, a typical lake in the cold and arid region of China, were studied by multiple methods. The interactions and fee7dback mechanisms between the sedimentary evolution of SOM and climate change, and compositions of SOM source change, were also discussed. Overall, the characteristic indexes of the SOM, including total organic carbon (TOC), carbon stable isotope (δ13C), carbon to nitrogen ratio (C/N), and fluorescence intensity (FI) of the protein-like component in water extractable organic matter (WEOM), showed obvious and uniform characteristics of periodical changes. The indexes were relatively stable before 1920, and fluctuated from 1920 to 1979. Since the 1980s, values of TOC, δ13C, and FI of the protein-like component in WEOM has increased, while C/N decreased. The absolute dominant contribution of terrestrial source to the SOM had changed, and the relative average contribution rate of autochthonous source increased from 17.6% before 1920 to 36.9% after 2000. The increase of temperature, strong evaporation concentration effect, and change of compositions of SOM sources are the important driving factors of the sedimentary evolution of organic matter in Hulun Lake.

The spatiotemporal distributions, influential factors, and ecological risks of 15 phthalate acid esters (PAEs) in the sediments of three typical lakes in Inner Mongolia were investigated in this work. PAEs were widespread in all surface sediments ranging from 598.3 to 3873.8 μg/kg dry weight. Average PAE concentrations were highest in the surface sediments of Wuliangsuhai Lake followed by Daihai and Hulun Lakes, which were consistent with population density and intensity of anthropogenic activities. Dibutyl phthalate, di-iso-butyl phthalate (DIBP), and diethyl phthalate (DEP) were the dominant PAEs with similar abundance orders for the three lakes. The cold climate could explain the higher DEP abundance in the sediments of lakes at higher latitudes. The highest Σ PAE and most individual PAE concentrations were located at one of the discharge river estuaries because most PAEs were terrestrial pollutants. Σ PAE concentrations in the core sediments increased with years and fluctuations owing to the increasing PAE usage and input. The second highest PAE concentration peaks were associated with the high gross domestic product growth rates in 2003-2008. Redundancy analysis revealed that butyl benzyl phthalate and diamyl phthalate in the surface sediments of Hulun Lake were principally positively influenced by organic matter (65.6% contribution among geochemical variables), Fe oxides (25.1% contribution among metals), and Mn oxides (23.8% contribution among metals). The DIBP and DEP exhibited high risks to sensitive fish according to a risk quotient assessment. This work presents new insights into PAE status and deposition mechanisms in the lakes within high latitudes or cold climates, and provides important information for future environmental protection and management.

Lakes in arid and semi-arid regions are experiencing dramatic variations in water level and volume, which has caused severe ecological and social problems. Long-term study of the lake dynamics in arid/semi-arid regions could provide particular insights into the mechanisms driving lake variations, while hydro-meteorological data were usually limited in these regions, especially before the instrumental period. In the present study, we focused on a typical great lake - Hulun Lake in semi-arid region in northern China, simulated the hydrological processes from 1904 to 2016 using SWAT model, CRUNCEP7 reanalysis data, and sparse records of lake level during 1900s-1950s, and investigated the mechanisms driving the dramatic variations of the lake at the hundred-year time scale. Results illustrated that the simplified Penman equation by Valiantzas (2006) could reproduce the evaporation dynamics of Hulun Lake, with monthly R2 being 0.93-0.95. The long-term simulation since 1904 reproduced runoff dynamics, which were consistent with the dramatic variations of lake level over hundred years. The largest water level increase (~5.0 m in 1950s) and decrease (~4.5 m in 2000s) during 1904-2016 were jointly affected by river runoff, lake evaporation, and precipitation into the lake. Both the positive/negative phase and the multi-decadal trend of PDO clearly influenced the hydrological cycle of Hunlun Lake, especially for the period of 1904-1950 with low lake levels. Overall, the present study provided a methodology for investigating the hundred-year hydrological processes for lakes in semi-arid regions in northeastern Asia.

Bacteria have the metabolic potential to produce a diverse array of secondary metabolites, which have important roles in biogeochemical cycling processes. However, for Hulun Lake and the rivers that enter into it, the bacterial community structures and their effects have not previously been widely studied, limiting our ecological understanding of this habitat. To address this, we have analyzed the bacterial communities in the water ecosystem of the Hulun Lake Basin. 16S rRNA high-throughput sequencing identified 64 phyla, 165 classes, 218 orders, 386 families, and 740 genera of bacteria across all samples. The dominant phyla in the central area of the lake were Proteobacteria, Actinobacteria, Firmicutes, and Cyanobacteria, while in all other areas, Proteobacteria, Actinobacteria, and Bacteroidetes were dominant. The microbial community structures were significantly affected by environmental factors [arsenic (As), pH, and sulfate (SO4 2-)] and their location in the lake. The species richness in the sediments of Hulun Lake was higher than in the water, and this ecosystem harbored the highest proportion of unclassified sequences, representing unclassified bacteria. This study provides basic data for future investigations into the Hulun lake ecosystem and for water microbial monitoring and protection measures.