PDF(Sci-hub)
Abstract：
Bacteria have the metabolic potential to produce a diverse array of secondary metabolites, which have important roles in biogeochemical cycling processes. However, for Hulun Lake and the rivers that enter into it, the bacterial community structures and their effects have not previously been widely studied, limiting our ecological understanding of this habitat. To address this, we have analyzed the bacterial communities in the water ecosystem of the Hulun Lake Basin. 16S rRNA high-throughput sequencing identified 64 phyla, 165 classes, 218 orders, 386 families, and 740 genera of bacteria across all samples. The dominant phyla in the central area of the lake were Proteobacteria, Actinobacteria, Firmicutes, and Cyanobacteria, while in all other areas, Proteobacteria, Actinobacteria, and Bacteroidetes were dominant. The microbial community structures were significantly affected by environmental factors [arsenic (As), pH, and sulfate (SO4 2-)] and their location in the lake. The species richness in the sediments of Hulun Lake was higher than in the water, and this ecosystem harbored the highest proportion of unclassified sequences, representing unclassified bacteria. This study provides basic data for future investigations into the Hulun lake ecosystem and for water microbial monitoring and protection measures.
摘要：
细菌具有产生多种次生代谢产物的代谢潜力，在生物地球化学循环过程中具有重要作用。然而,呼伦湖和进入它的河流，细菌群落结构及其影响以前没有被广泛研究，限制了我们对这个栖息地的生态理解。为了解决这个问题，我们分析了呼伦湖流域水生态系统中的细菌群落。16SrRNA高通量测序鉴定出64门，165类,218个订单,386个家庭,和所有样本中的740属细菌。湖泊中部的优势门是变形杆菌，放线菌,Firmicutes,和蓝细菌，而在所有其他领域，变形杆菌,放线菌,和拟杆菌占优势。微生物群落结构受环境因素[砷（As），pH值,和硫酸盐（SO42-）]及其在湖中的位置。呼伦湖沉积物中的物种丰富度高于水中，这个生态系统拥有最高比例的未分类序列，代表未分类的细菌。本研究为今后开展呼伦湖生态系统调查和水体微生物监测与保护措施提供了基础数据。
