最近,HexNAcQuest被开发用于帮助区分由HexNAc异构体修饰的肽，更具体地说，O-连接的β-N-乙酰葡糖胺（O-GlcNAc）和O-连接的α-N-乙酰半乳糖胺（O-GalNAc，Tn抗原）。为了促进其使用（特别是对于来自糖蛋白质组学研究的数据集），在这里，我们提出了一个详细的协议。它描述了用户可能需要使用HexNAcQuest来区分这两个修改的示例案例和过程。
Recently,
HexNAcQuest was developed to help distinguish peptides modified by HexNAc isomers, more specifically O-linked β-N-acetylglucosamine (O-GlcNAc) and O-linked α-N-acetylgalactosamine (O-GalNAc, Tn antigen). To facilitate its usage (particularly for datasets from glycoproteomics studies), herein we present a detailed protocol. It describes example cases and procedures for which users might need to use
HexNAcQuest to distinguish these two modifications.
