紫菜含有大量的生物活性化合物,这些化合物被合成并储存在它们的油体中。然而,关于单个物种化学成分的知识仍然不完整。这项研究的主题是Calypogeiaintegristipula,一种代表多叶的苔藓的物种。从波兰的各个地方收集了用于化学分类学研究的植物材料。在2021年和2022年三个生长季节从自然环境中收集的74个样品中确定了化学成分:春季,夏天和秋天,并与源自体外培养的样品进行比较。根据形态特征将植物分类为Calypogeiaintegristipula,油体,和DNA标记。通过顶空固相微萃取(HS-SPME)从生物材料中提取挥发性有机化合物(VOC)。然后通过气相色谱-质谱(GC-MS)分析样品。共检测到79个化合物,其中鉴定出44种化合物。使用MS断裂谱描述剩余的化合物。观察到与Calypogeiaintegristipula生长季节相关的化合物组成的周期性变化。此外,来自体外培养的样品和来自自然环境的样品显示出化合物的组成不同。在数量上,在挥发性化合物中,属于倍半萜类(46.54-71.19%)和倍半萜类(8.12-22.11%)的化合物占主导地位。少数化合物属于芳香族化合物(2.30-10.96%),单萜(0.01-0.07%),单萜(0.02-0.33%),和脂肪烃(1.11-6.12%)。所分析的紫草中的主要化合物是:anastreptene(15.27-31.14%);双环醇(6.99-18.09%),4,5,9,10-脱氢-异戊烯(2.00-8.72%),palustrol(4.95-9.94%),spathulenol(0.44-5.11%)。
Liverworts contain a large number of biologically active compounds that are synthesised and stored in their oil bodies. However, knowledge about the chemical composition of individual species is still incomplete. The subject of the study was Calypogeia integristipula, a species representing leafy liverworts. Plant material for chemotaxonomic studies was collected from various locations in Poland. The chemical composition was determined in 74 samples collected from the natural environment in 2021 and 2022 in three growing seasons: spring, summer and autumn, and for comparison with samples originating from in vitro culture. The plants were classified as Calypogeia integristipula on the basis of morphological characteristics, oil bodies, and DNA markers. The volatile organic compounds (VOCs) from the biological material were extracted by headspace solid phase microextraction (HS-SPME). The samples were then analysed by gas chromatography-mass spectrometry (GC-MS). A total of 79 compounds were detected, of which 44 compounds were identified. The remaining compounds were described using the MS fragmentation spectrum. Cyclical changes in the composition of compounds associated with the growing season of Calypogeia integristipula were observed. Moreover, samples from in vitro culture and samples taken from the natural environment were shown to differ in the composition of chemical compounds. In terms of quantity, among the volatile compounds, compounds belonging to the sesquiterpene group (46.54-71.19%) and sesqiuterpenoid (8.12-22.11%) dominate. A smaller number of compounds belong to aromatic compounds (2.30-10.96%), monoterpenes (0.01-0.07%), monoterpenoids (0.02-0.33%), and aliphatic hydrocarbons (1.11-6.12%). The dominant compounds in the analysed liverworts were: anastreptene (15.27-31.14%); bicyclogermacrene (6.99-18.09%), 4,5,9,10-dehydro-isolongifolene (2.00-8.72%), palustrol (4.95-9.94%), spathulenol (0.44-5.11%).