中国刺,也被称为清波,是中国重要的经济鱼类。然而,其增长背后的详细机制仍然未知。挖掘与其生长相关的基因和信号通路,我们比较了中华链球菌肝胰腺组织的转录组概况,用两组生长速度进行评价。在快速生长(FG)和缓慢生长(SG)组中平均获得了66,304,909和68,739,585个清洁读数,分别。差异基因表达分析结果显示,在FG和SG组间筛选出272个差异表达基因(DEGs),包括101个上调基因和171个下调基因。基因本体论(GO)和京都基因百科全书(KEGG)富集分析结果表明,GO术语与代谢过程有关,有机物代谢过程,催化活性得到了丰富,还检测了与类固醇生物合成和蛋白质消化吸收相关的通路信号。同时,筛选了与中华黄连生长相关的潜在关键调控基因sst2、fndc4和cckra。逆转录荧光定量PCR(RT-qPCR)对18个与生长差异相关的DEGs进行验证,结果表明RT-qPCR结果与RNA-seq分析结果一致,和九个基因,stk31、gpr149、angptl1、fstl1、sik1、ror2、nlrc3、pdlim2和nav2在FG组中显著表达。bmp1、stc1、gpatch8、sstrt2、s100a1、ktf6、cckar6、sync1、bhlha15等共9个基因在SG组中显著表达。本研究为改善中华黄连的生长特性和候选基因功能研究提供了基础资料。
Spinibarbus sinensis, also known as Qingbo, is an important economic fish in China. However, the detailed mechanisms underlying its growth are still unknown. To excavate the genes and signaling pathways related to its growth, we compared the transcriptome profiles of the
hepatopancreas tissues of S. sinensis, with two groups of growth rate for evaluation. An average of 66,304,909 and 68,739,585 clean reads were obtained in the fast growth (FG) and slow growth (SG) group, respectively. The differential gene expression analysis results showed that 272 differentially expressed genes (DEGs) were screened between the FG and SG groups, including 101 up-regulated genes and 171 down-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results showed that GO terms related to metabolic process, organic substance metabolic process, and catalytic activity were enriched, pathway signals related to steroid biosynthesis and protein digestion and absorption were also detected. Meanwhile, the potential key regulatory genes sst2, fndc4, and cckra related to the growth of S. sinensis were screened. Reverse transcript fluorescence quantitative PCR (RT-qPCR) validation of 18 DEGs associated with growth differences showed that the RT-qPCR results were consistent with RNA-seq analysis, and nine genes, stk31, gpr149, angptl1, fstl1, sik1, ror2, nlrc3, pdlim2, and nav2 were significantly expressed in the FG group. bmp1, stc1, gpatch8, sstrt2, s100a1, ktf6, cckar6, sync1, bhlha15, a total of nine genes were significantly expressed in the SG group. This study provides basic information for improving the growth characteristics of S. sinensis and the functional research of candidate genes.