严重发热伴血小板减少综合征(SFTS)病毒和汉坦病毒按Bunyavirales顺序分类。SFTS和肾综合征出血热(HFRS)的严重疾病进展与细胞因子风暴有关。本研究旨在探讨两种疾病之间细胞因子谱和免疫反应的差异。横截面,单中心研究涉及100名参与者,包括46名SFTS患者,48例HFRS患者,和6个健康对照。该研究采用Luminex细胞因子检测平台来测量48种细胞因子。进一步采用多元线性回归分析两种疾病之间细胞因子谱和免疫特征的差异,主成分分析,和随机森林方法。在测试的48种细胞因子中,与健康对照组相比,图30显示SFTS和/或HFRS的水平升高。此外,有19种细胞因子在SFTS和HFRS之间表现出显着差异。随机森林分析表明TRAIL和CTACK对SFTS有预测作用,而IL2Ralpha,MIG,IL-8,IFNα2,HGF,SCF,MCP-3和PDGFBB在HFRS中更常见。除TRAIL外,曲线下面积>0.8且P值<0.05的接收器工作特性进一步验证了这一点。在SFTS和HFRS的细胞因子谱中观察到显著差异,有了TRAIL,IL2Ralpha,MIG,和IL-8是最清楚区分这两种疾病的前4种细胞因子。
目的:SFTS和HFRS在细胞因子免疫特性方面存在差异。TRAIL,IL-2Rα,MIG,IL-8是SFTS和HFRS之间差异显着的前4位。
Severe fever with thrombocytopenia syndrome (SFTS) virus and hantavirus are categorized under the Bunyavirales order. The severe disease progression in both SFTS and hemorrhagic fever with renal syndrome (HFRS) is associated with cytokine storms. This study aimed to explore the differences in cytokine profiles and immune responses between the two diseases. A cross-sectional, single-center study involved 100 participants, comprising 46 SFTS patients, 48 HFRS patients, and 6 healthy controls. The study employed the Luminex cytokine detection platform to measure 48 cytokines. The differences in cytokine profiles and immune characteristics between the two diseases were further analyzed using multiple linear regression, principal component analysis, and random forest method. Among the 48 cytokines tested, 30 showed elevated levels in SFTS and/or HFRS compared to the healthy control group. Furthermore, there were 19 cytokines that exhibited significant differences between SFTS and HFRS. Random forest analysis suggested that TRAIL and CTACK were predictive of SFTS, while IL2Ralpha, MIG, IL-8, IFNalpha2, HGF, SCF, MCP-3, and PDGFBB were more common with HFRS. It was further verified by the receiver operating characteristic with area under the curve >0.8 and P-values <0.05, except for TRAIL. Significant differences were observed in the cytokine profiles of SFTS and HFRS, with TRAIL, IL2Ralpha, MIG, and IL-8 being the top 4 cytokines that most clearly distinguished the two diseases.
OBJECTIVE: SFTS and HFRS differ in terms of cytokine immune characteristics. TRAIL, IL-2Ralpha, MIG, and IL-8 were the top 4 that differed markedly between SFTS and HFRS.