背景:香菜是一种传统的药用和食用植物。它富含类黄酮化合物,是一种具有多种保健功效和药用价值的重要生物活性成分。然而,黄酮类代谢组学概况和不同部位黄酮类化合物的比较很少。
结果:在这项研究中,从根研究黄酮类代谢物,茎,H.citrina的叶子和花。通过基于UPLC-MS/MS的广泛靶向代谢组学,共鉴定了364种类黄酮代谢物,四个植物部分在黄酮代谢水平上表现出巨大差异。与根相比,185,234和119代谢物占茎中差异类黄酮代谢物(DFM)的上调,叶子,和鲜花,分别。与茎相比,168和29类黄酮代谢产物在叶和花中占上调的DFM,分别。与树叶相比,花中只有29种类黄酮代谢产物被上调。在六个对照组中观察到35种常见的类黄酮代谢产物,每个对照组都有其独特的差异代谢物。四个部分中最丰富的类黄酮代谢产物是黄酮醇和黄酮,其次是黄烷酮,查尔酮,黄烷醇,黄烷醇,花青素,单宁,和原花青素。6,7,8-四羟基-5-甲氧基黄酮,7,8,3\',4\'-四羟基黄酮,1-羟基-2,3,8-三甲氧基黄吨酮,Farrerol-7-O-葡萄糖苷,3\',7-二羟基-4'-甲氧基黄酮,3,3'-O-二甲基鞣花酸,5-羟基-6,7-二甲氧基黄酮,内佩汀(5,7,3\',4'-四羟基-6-甲氧基黄酮),(2s)-4,8,10-三羟基-2-甲氧基-1h,2h-furo[3,2-a]xanthen-11-one在根中占主导地位。异鼠李素-3-O-(6'-丙二酰)葡糖苷-7-O-鼠李糖苷,7-苄氧基-5-羟基-3',4'-亚甲二氧基类黄酮,3-羟基孕酮-4'-O-葡萄糖苷在茎中占优势。Chrysoeriol-7-O-葡萄糖苷,表儿茶素葡萄糖苷,山奈酚-3-O-鼠李糖苷(Afzelin)(Kaempferin)*,Azaleatin(5-O-甲基槲皮素),Chrysoeriol-5-O-葡萄糖苷,Nepetin-7-O-葡萄糖苷(Nepitrin),3,5,7,2'-四羟基黄酮;地皮汀,原花青素B2*,原花青素B3*,原花青素B1,异鼠李素-3-O-(6\'\'-乙酰葡糖苷)在叶片中占主导地位。山奈酚-3-p-香豆酰基二葡萄糖苷,Delphinidin-3-O-槐苷-5-O-葡萄糖苷,Limocitrin-3-O-苦参,山奈酚-3-O-鲁丁苷(烟草素),木犀草素-7-O-(6\'\'-丙二酰)葡糖苷-5-O-鼠李糖苷在花中占优势。
结论:不同部位的黄酮类代谢产物存在显著差异。叶片的代谢物含量相对高于其他部分。这项研究提供了生物和化学证据的各种植物部分的不同用途。这些信息是食品工业的重要理论基础,和医疗。
BACKGROUND: Hemerocallis citrina Baroni is a traditional medical and edible plant. It is rich in flavonoid compounds, which are a kind of important bioactive components with various health benefits and pharmaceutical value. However, the flavonoid metabolomics profile and the comparison of flavonoid compounds from different parts of H. citrina is scarce.
RESULTS: In this study, flavonoid metabolites were investigated from roots, stems, leaves and flowers of H. citrina. A total of 364 flavonoid metabolites were identified by UPLC-MS/MS based widely targeted metabolomics, and the four plant parts showed huge differences at flavonoid metabolic level. Compared to roots, 185, 234, and 119 metabolites accounted for upregulated differential flavonoid metabolites (DFMs) in stems, leaves, and flowers, respectively. Compared to stems, 168 and 29 flavonoid metabolites accounted for upregulated DFMs in leaves and flowers, respectively. Compared to leaves, only 29 flavonoid metabolites accounted for upregulated DFMs in flowers. A number of 35 common flavonoid metabolites were observed among six comparison groups, and each comparison group had its unique differential metabolites. The most abundant flavonoid metabolites in the four parts are flavonols and flavones, followed by flavanones, chalcones, flavanols, flavanonols, anthocyanidins, tannin, and proanthocyanidins. 6,7,8-Tetrahydroxy-5-methoxyflavone, 7,8,3\',4\'-tetrahydroxyflavone, 1-Hydroxy-2,3,8-trimethoxyxanthone, Farrerol-7-O-glucoside, 3\',7-dihydroxy-4\'-methoxyflavone, 3,3\'-O-Dimethylellagic Acid, 5-Hydroxy-6,7-dimethoxyflavone, Nepetin (5,7,3\',4\'-Tetrahydroxy-6-methoxyflavone), (2s)-4,8,10-trihydroxy-2-methoxy-1 h,2 h-furo[3,2-a]xanthen-11-one are dominant in roots. Isorhamnetin-3-O-(6\'\'-malonyl)glucoside-7-O-rhamnoside, 7-Benzyloxy-5-hydroxy-3\',4\'-methylenedioxyflavonoid, 3-Hydroxyphloretin-4\'-O-glucoside are dominant in stems. Chrysoeriol-7-O-glucoside, Epicatechin glucoside, Kaempferol-3-O-rhamnoside (Afzelin)(Kaempferin)*, Azaleatin (5-O-Methylquercetin), Chrysoeriol-5-O-glucoside, Nepetin-7-O-glucoside(Nepitrin), 3,5,7,2\'-Tetrahydroxyflavone; Datiscetin, Procyanidin B2*, Procyanidin B3*, Procyanidin B1, Isorhamnetin-3-O-(6\'\'-acetylglucoside) are dominant in leaves. kaempferol-3-p-coumaroyldiglucoside, Delphinidin-3-O-sophoroside-5-O-glucoside, Limocitrin-3-O-sophoroside, Kaempferol-3-O-rutinoside(Nicotiflorin), Luteolin-7-O-(6\'\'-malonyl)glucoside-5-O-rhamnoside are dominant in flowers.
CONCLUSIONS: There was significant difference in flavonoid metabolites among different parts of H. citrina. Leaves had relative higher metabolites contents than other parts. This study provided biological and chemical evidence for the different uses of various plant parts of H. citrina, and these informations are important theoretical basis for the food industry, and medical treatment.